Figure 5.

Effects of SIDT2 knockdown on RNA uptake and degradation by lysosomes. (A and D) Decreased levels of SIDT2 proteins in HeLa cells transfected with SIDT2-siRNA were confirmed by immunoblotting. Relative levels of SIDT2 were quantified. Results are expressed as mean ± SEM (n = 3). (B and E) RNA uptake assay I (Fig. 2A) was performed using isolated lysosomes derived from SIDT2 knockdown or control siRNA-transfected cells. Relative levels of RNA uptake were quantified. Mean ± SEM (n = 3). ***, P < 0.001. (C and F) RNA degradation assay using lysosomes isolated from SIDT2-knockdown cells or from control cells. Relative levels of RNA degradation were quantified. Mean ± SEM (n = 3). ***, P < 0.001; **, P < 0.01.