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. Author manuscript; available in PMC: 2016 Apr 19.
Published in final edited form as: Nature. 2016 Mar 16;531(7595):471–475. doi: 10.1038/nature17157

Extended Data Figure 8. Alox15b deficiency promotes tumorigenesis and increases AA levels.

Extended Data Figure 8

a, Enrichment fold of shAlox15b.1252 and shAlox15b.2865 in resulting tumours (Fig. 3i, j) compared to those in initiating shRNA libraries. b, Knockdown efficiency of shAlox15b.1252 and shAlox15b.2865 compared to control shRen in NIH3T3 cells, as detected by western blotting and quantitated by ImageJ. c, Relative levels of AA per cell are increased with Alox15b shRNAs as measured by liquid chromatography–mass spectrometry (LC-MS). NIH3T3 cells were transduced with shRen or shAlox15b. n = 3. **P < 0.01 (unpaired two tailed t-test). d, Relative levels of AA per cell in 11B3Δ/Trp53Δframeshift (11B3) lymphoma cells are higher than control cells with Trp53Δ/Δ (p53) as measured by LC-MS. n = 2. P = 0.056 (unpaired two tailed t-test). e, In vitro AA treatments reduce apoptosis, as measured by annexin V staining of pre-B cells after 20 h treatment of indicated concentration of AA. n = 4. *P < 0.05; ***P < 0.001 (unpaired two tailed t-test).