FIGURE 3.

In vivo stabilization activity of MALAT1 derivatives with quadruple base-triple substitutions. (A) Constructs containing the MALAT1 ENE + A + mascRNA and derivatives thereof were prepared and introduced into HEK293T cells as described in Figure 2. (B) The base composition at each of the four sites boxed in blue was varied to test the stabilization activity of selected RNA base triples as a complete, independent triple-helical unit. Structural notation is as described in Figure 1. (C) Total RNA was extracted and analyzed as described in Figure 2. Measurements are an average of at least three biological replicates ± standard deviation.