Abstract
Mononuclear nonheme iron(III)-superoxo species (FeIII-O2−•) have been implicated as key intermediates in the catalytic cycles of dioxygen activation by nonheme iron enzymes. Although nonheme iron(III)-superoxo species have been trapped and characterized spectroscopically in enzymatic and biomimetic reactions, no structural information has yet been obtained. Here we report for the first time the isolation, spectroscopic characterization, and crystal structure of a mononuclear side-on (η2) iron(III)-superoxo complex with a tetraamido macrocyclic ligand (TAML), [FeIII (TAML) (O2)]2− (1). The nonheme iron(III)-superoxo species undergoes both electrophilic and nucleophilic oxidation reactions as well as O2-transfer between metal complexes. In the O2-transfer reaction, 1 transfers the bound O2 unit to a manganese(III) analogue, resulting in the formation of a manganese(IV)-peroxo complex, [MnIV(TAML)(O2)]2− (2); 2 is characterized structurally and spectroscopically as a mononuclear side-on (η2) manganese(IV)-peroxo complex. The difference in the redox distribution between the metal ions and O2 in 1 and 2 is rationalized using density functional theory calculations.
Introduction
Metal-dioxygen adducts (M-O2), such as metal-superoxo and metal-peroxo species, are invoked as key intermediates in the transport, storage, and activation of dioxygen (O2) by metal-containing proteins and enzymes as well as in O2-formation by Photosystem II1–4. The structures and spectroscopic properties of O2-binding metal complexes have been investigated intensively over the past half-century, and several crystal structures of O2-binding iron species have been reported in heme and nonheme iron enzymes and their model compounds5–10. One of the first reported crystal structures of an O2-bound iron intermediate was obtained using a synthetic heme complex as a model of oxy-hemoglobin and oxy-myoglobin8. The structural and spectroscopic characterization of the O2-binding iron intermediates in heme-containing proteins and synthetic iron porphyrins revealed that these iron(III)-superoxo species bind the O2 unit in an end-on fashion7–9. In contrast, crystal structures of iron-O2 intermediates in nonheme iron enzymes and models revealed that the O2 unit in iron(III)-peroxo species binds to the iron center in a side-on fashion5, 6, 10; the crystal structure of a mononuclear nonheme iron(III)-peroxo complex, [FeIII(TMC)(O2)]+, TMC = 1,4,8,11-tetramethyl-1,4,8,11-tetraazacyclotetradecane), shows the binding of O2 unit in a side-on fashion with an O-O bond distance of 1.463(6) Å10. Another example is the structure of naphthalene dioxygenase which shows an O2 unit bound in a side-on fashion6. However, to the best of our knowledge, no structural information has been obtained for nonheme iron(III)-superoxo species, although such intermediates have been proposed under catalytic reaction conditions and trapped and characterized spectroscopically in nonheme iron enzymes and models11–13. For example, Nam and co-workers proposed the short-lived “putative” Fe(III)-superoxo intermediate during the dioxygen activation by nonheme iron(II) complexes while Goldberg and co-workers demonstrated that the binding sulfur ligand at the axial position iron(II) complex facilitated the O2 binding, therefore, generating iron(III)-superoxo species. However, none of these showed direct structural evidence of the iron(III)-superoxo species.14–16 Further, although nonheme iron(III)-superoxo intermediates have been proposed as reactive species in electrophilic oxidation reactions by nonheme iron complexes, direct evidence for the involvement of such nonheme iron(III)-superoxo species in electrophilic oxidation reactions has not been reported in nonheme iron models17, 18. Furthermore, the involvement of nonheme iron(III)-superoxo species in nucleophilic oxidation reactions has been rarely discussed in either enzymatic or biomimetic reactions. Thus, understanding the structural information and chemical properties, in particular the oxidation reactivity, of iron(III)-superoxo species has become an important objective for bioinorganic chemists.
We now report for the first time the synthesis, spectroscopic and electronic properties, and crystal structure of a mononuclear side-on (η2) iron(III)-superoxo complex bearing tetraamido macrocyclic ligand (TAML)19, [FeIII(TAML)(O2)]2− (1) (Fig. 1a; see also Supplementary Fig. 1 and Supplementary Table 1). The isolated iron(III)-superoxo complex undergoes both electrophilic and nucleophilic oxidation reactions. In addition, the iron(III)-superoxo complex transfers the bound O2 unit to a manganese(III) analogue, resulting in the formation of a mononuclear side-on (η2) manganese(IV)-peroxo complex, [MnIV(TAML)(O2)]2− (2) (Fig. 1b; see also Supplementary Fig. 2 and and Supplemetary Table 2). The electronic structures of 1 and 2 are investigated using density functional theory (DFT) calculations to elucidate the difference in the redox distribution between the metal ion and O2 in these complexes.
Results and discussion
Synthesis and structural and spectroscopic characterization of iron(III)-superoxo complex
The reaction of Na[FeIII(TAML)] with solid potassium superoxide (KO2) in the presence of 6 equiv. of 2.2.2-cryptand in CH3CN at 5 °C immediately generated a red intermediate (1) with an electronic absorption band at λmax = 490 nm (ε = 2600 M−1 cm−1) (Fig. 2a; Supplementary Experimental Section). The intermediate persisted for several days at −20 °C, and the greater thermal stability of 1 allowed us to isolate single crystals suitable for structural and spectroscopic analyses as well as for reactivity studies. The electrospray ionization mass spectrum (ESI MS) of 1 exhibits a prominent ion peak at a mass-to-charge (m/z) ratio of 873.1, whose mass and isotope distribution pattern correspond to [K(2.2.2-cryptand)Fe(TAML)(O2)]− (calculated m/z of 873.3) (Fig. 2a, inset; also see Supplementary Fig. 3a). When the reaction was carried out with isotopically labeled K18O2, a mass peak corresponding to [K(2.2.2-cryptand)Fe(TAML)(18O2)]− appeared at a m/z of 877.1 (calculated m/z of 877.3). The shift in four mass units on substitution of 16O with 18O indicates that 1 contains an O2 unit (Fig. 2a, inset). In the electron paramagnetic resonance (EPR) spectrum of a frozen acetonitrile solution of 1 recorded at 4.3 K, no signal was observed in both perpendicular and parallel modes (Supplementary Fig. 3b), which might arise from the electronic configuration of an iron(IV)-peroxo species (S = 1 or 2) or an iron(III) species (S = 1/2, 3/2, or 5/2) coupled with an O2− unit ofS = 1/2. The electronic configuration of 1 was further investigated with 1H NMR Evans method, and the magnetic moment of 3.26 µB at −20 °C is consistent with an S = 1 ground state for 1. Based on those experimental results, there are three possible structures for 1: (i)an Fe(IV)-peroxo species with S = 1 Fe(IV), (ii) an Fe(III)-superoxo species with S = 1/2 Fe(III) ferromagnetically coupled with S = 1/2 O2− moiety, or (iii) an Fe(III)-superoxo species with S = 3/2 Fe(III) antiferromagnetically coupled with an S = 1/2 O2− moiety (vide infra). The infrared spectrum of 1, collected in CH3CN at −40 °C, exhibits an isotopically sensitive band at 1260 cm−1, which shifts to 1183 cm−1 upon substitution of 16O with 18O, consistent with its assignment as an O-O stretching vibration on the basis of the 16–18Δ value of 77cm−1 (16–18Δ (calculated) = 72 cm−1) (Fig. 2b). This value is comparable to those recorded for metal-superoxo complexes9, 13, 20, 21, suggesting that the O2 unit in 1 possesses superoxo character.
Mössbauer spectroscopy was also performed to determine the oxidation state of iron in 1 and define its electronic structure (Fig. 2c, δ = 0.10 mm s−1 and ΔEQ = 2.66 mm s−1). The isomer shift is in the same range as that for TAML complexes with S = 3/2 Fe(III) centers22. Following the procedure of Chanda et al.22, the density at the nucleus ρ and ΔEQ for this complex were calculated using the 6-311G basis set and B3LYP functional (Supplementary Experimental Section); the density at the nucleus was converted into an isomer shift using the calibration of Vrajmasu et al23. The computational isomer shift obtained using this method, δ = 0.12 mm s−1, and the calculated ΔEQ, 2.78 mm s−1, are in excellent agreement with the experimental values. Thus, the spectroscopic data described above suggest that 1 is a mononuclear iron(III) species binding a superoxo ligand, FeIII-O2−•.
Direct evidence for an Fe–O2 unit is unambiguously provided from the X-ray crystal structure of 1, which revealed a mononuclear side-on 1:1 iron complex of O2 with the triangular Fe–O2 moiety having a small bite angle (40.37(9)° for O1-Fe1-O2, 39.9(2)° for O7-Fe2-O8, 39.4(4)° for O7’-Fe2-O8’), that results in a pseudo square pyramidal geometry with side-on biding O2 moiety (Fig. 1a, also see Supplementary Fig. 1 and Supplemetary Table 1). The crystallographically independent O–O bond lengths (O1-O2: 1.323(3) Å, O7-O8/O7’-O8’ = 1.306(7)/1.315(12) Å) of 1 are significantly shorter than those of Fe(III)-peroxo species, such as [FeIII(TMC)(O2)]+ (1.463(6) Å)10 and naphthalene dioxygenase (ca. 1.45 Å)6, but similar to that of Fe(II)-superoxo species found in homoprotocatechuate 2,3-dioxygenase (1.34 Å)5. Further, the observed O-O bond length is close to those of other metal-superoxo complexes that have been characterized structurally (ca. 1.2–1.3 Å)9, 21. Furthermore, the O2 ligand within Fe-O2 unit is almost symmetrically bound to the iron ion with the average Fe–O bond length of 1.927 Å, which is slightly longer than that of [FeIII(TMC)(O2)]+ (1.910 Å). In accordance with these experimental values, we conclude that 1 is an iron(III)-superoxo complex with the O2 unit bound in a side-on fashion (η2). To the best of our knowledge, this is the first X-ray crystal structure of a mononuclear nonheme iron(III)-superoxo complex reported in nonheme iron enzyme and model systems.
Reactivity studies of 1 in electrophilic and nucleophilic oxidation and O2-transfer
As presented in the Introduction, nonheme iron(III)-superoxo species have attracted much attention recently as active oxidants in a number of enzymatic reactions, especially in electrophilic oxidation reactions by isopenicillin N synthase, myo-inositol oxygenase, and cysteine dioxygenase2, 24, 25. However, nucleophilic reactivity of the nonheme iron(III)-superoxo species has not been previously reported. Moreover, reactivities of synthetic nonheme iron(III)-superoxo complexes in electrophilic and nucleophilic oxidation reactions have been rarely reported in biomimetic studies.20 We therefore performed these reactions with the isolated, well-characterized nonheme iron(III)-superoxo complex, 1. First, the reactivity of 1 was examined in the oxidation of C-H and O-H bonds, with precedents that metal-superoxo species are potent oxidants in the C-H and O-H substrate oxygenation reactions11, 26–28. Addition of substrates with weak C-H bonds, such as cyclohexadiene and xanthene, to 1 did not show any spectral changes, indicating that 1 is not capable of activating the C-H bonds of hydrocarbons. In the case of O-H bond activation, addition of 2,4-di-tert-butylphenol to 1 caused the decay of the intermediate with the concurrent formation of [FeIII(TAML)]− (Supplementary Fig. 4a). Clear isosbestic points were observed at 333 and 393 nm. The decay rate increased with increase of the substrate concentration, giving a second-order rate constant of 3.0×10−1 M−1 s−1 (Supplementary Fig. 4b). Product analysis of the reaction solution revealed the formation of 2,2’-dihydroxy-3,3’, 5,5’-tetra-tert-butylbiphenol as a major product (~75% based on the intermediates used)29, 30. Further, reactions with para-substituted 2,6-di-tert-butylphenols (p-Y-2,6-t-Bu2-C6H2OH; Y = OMe, Me, H, CN) revealed that the electronegativity of the para-substituents significantly influences the reaction rates (Supplementary Fig. 5); a plot of the relative rates as a function of O-H bond dissociation energies (BDEs) of p-Y-2,6-t-Bu2-C6H2OH shows a good correlation with a slope of –0.65 (Supplementary Fig. 4c). In high-valent metal-oxo and metal-superoxo complexes, a linear relationship such as this has been cited in support of an H-atom abstraction mechanism in phenol O-H bond oxidations29–31.
The nucleophilic reactivity of 1 was investigated in aldehyde deformylation, using 2-phenylpropionaldehyde (2-PPA) as a substrate. Upon addition of 2-PPA to 1 in CH3CN at 5 °C, 1 was converted to the starting [FeIII(TAML)]− complex with a first-order decay profile(Supplementary Fig. 6a), and pseudo-first-order rate constants that increased proportionally with the aldehyde concentration (k2 = 3.7 M−1 s−1) (Supplementary Fig. 6b). Product analysis of the resulting solutions revealed the formation of acetophenone (90% based on 1), as frequently observed in other nucleophilic oxidation reactions by metal-peroxo complexes32, 33. The reactivity of 1 was further investigated using substituted benzaldehydes with a series of electron-donating and -withdrawing substituents at the para-position of the phenyl group (para-Y-Ph-CHO; Y = OMe, Me, H, Cl) (Supplementary Fig. 7). A positive ρ+ value of 1.4 in the Hammett plot was obtained (Supplementary Fig. 6c), demonstrating that the iron(III)-superoxo complex is an active nucleophilic oxidant; the nucleophilic reactivity of a copper(II)-superoxo complex was reported very recently34. To the best of our knowledge, the present results provide the first strong evidence for the involvement of iron(III)-superoxo species in nucleophilic oxidation reactions.
The reactivity of 1 was further investigated in an intermolecular O2 transfer reaction (Fig. 3a), with precedents that metal-peroxo species {e.g., [NiIII(12-TMC)(O2)]+, [CoIII(12-TMC)(O2)]+, and [CoIII(13-TMC)(O2)]+} are capable of transferring their peroxo group to a manganese complex [MnII(14-TMC)]2+ to give a manganese-peroxo complex {e.g., [MnIII(14-TMC)(O2)]+}35, 36. Interestingly, addition of [MnIII(TAML)]− to a solution containing 1 immediately generated a blue intermediate (2) with an electronic absorption band at 600 nm (Fig. 3b); the blue intermediate 2 was characterized as a mononuclear side-on (η2) manganese(IV)-peroxo complex (vide infra). Clear isosbestic points were observed at 462 and 561 nm in the titration reaction (Fig. 3b; Supplementary Fig. 8), in which the O2-transfer from 1 to [MnIII(TAML)]− was found to have 1:1 reaction stoichiometry (Fig. 3b, inset). Kinetic studies of the O2-transfer from 1 to [MnIII(TAML)]− were performed in DMF at −20 °C under pseudo-first-order reaction conditions. Addition of 10 equiv. of [MnIII(TAML)]− to the solution of 1 yielded kobs value of 1.0×10−2 s−1 (Supplementary Fig. 9a), with the first-order rate constant increasing proportionally with the concentration of [MnIII(TAML)]− that gives a second-order rate constant (k2) of 9.0×10−1 M−1 s−1 at –20 °C (Supplementary Fig. 9b). The intermolecular O2-transfer reaction was dependent on temperature, from which a linear Eyring plot was obtained between –20 and 10 °C to give activation parameters of ΔHǂ = 55 kJ mol−1 and ΔSǂ = –33 J mol−1 K−1 (Supplementary Fig. 10). The temperature dependence of the rate constants and the significant negative entropy value suggest that a bimolecular mechanism is operating in the O2-transfer reaction, where the formation of a presumed [(TAML)Fe-O2-Mn(TAML)]3− intermediate is the rate-determining step35, 36. Finally, the reverse reaction, which is the O2-transfer from 2 to [FeIII(TAML)]− to form [MnIII(TAML)]− and 1, does not occur.
In this section, we have shown that the nonheme iron(III)-superoxo complex, 1, is an active oxidant in both electrophilic and nucleophilic reactions. We have also shown that the O2 unit in the iron(III)-superoxo complex is transferred to a Mn(III)complex. Detailed mechanistic studies are underway to understand the chemical properties and mechanisms of nonheme iron(III)-superoxo species in oxidation reactions.
Synthesis and structural and spectroscopic characterization of manganese(IV)-peroxo complex
The manganese(IV)-peroxo complex, [MnIV(TAML)(O2)]2– (2), was synthesized independently by reacting Li[MnIII(TAML)] with solid KO2 in the presence of 6 equiv. of 2.2.2-cryptand in CH3CN at 25 °C (see Supplementary Fig. 11 and 12 and Supplementary Table 3 for the spectroscopic and structural characterization of [MnIII(TAML)]−). The high stability of 2 at room temperature allowed us to isolate single crystals suitable for structural and spectroscopic characterization. The UV-vis spectrum of 2 exhibits an absorption band at 600 nm (ε = 720 M−1 cm−1) (Supplementary Fig. 13a). The ESI MS of 2 exhibits a prominent ion peak at a mass-to-charge (m/z) ratio of 872.1, and its isotopic distribution pattern was in good agreement with [K(2.2.2-cryptand)Mn(TAML)(16O2)]− (calculated m/z of 872.3) (Supplementary Fig. 13b). When 2 was prepared with isotopically labeled K18O2, a mass peak corresponding to [K(2.2.2-cryptand)Mn(TAML)(18O2)]− appeared at a m/z of 876.1 (calculated m/z of 876.3). The shift in four mass units on substitution of 16O with 18O indicates that 2 contains an O2 unit (Supplementary Fig. 13b, inset). The EPR spectrum of 2 exhibits one six-line hyperfine pattern centered at g = 5.6 with splitting of a = 55 G and two broad signals at g = 2.3 and 1.7, indicating an S = 3/2 Mn(IV) ground state (Supplementary Fig. 13c)37, 38. The solid IR spectrum (KBr) of 2 exhibits an isotopically sensitive band at 905 cm−1, which shifts to 860 cm−1 upon substitution of 16O with 18O2, consistent with its assignment as an O-O stretching vibration on the basis of the 16–18Δ value of 45 cm−1 (16–18Δ (calculated) = 52 cm−1) (Supplementary Fig. 14). This value is comparable to those recorded for metal-peroxo complexes38, 39, thus leading us to conclude that the O2 unit in 2 possesses peroxo character.
The X-ray crystal structure of 2 is given in Fig.1b (also see Supplementary Fig. 2 and Supplementary Table 3). Although complex 2 also contains a mononuclear side-on 1:1 Mn–O2 moiety best described a pseudo square pyramidal geometry with small acute angles of 44.30(7)° for O1-Mn1-O2, 44.17(11)° for O7-Mn2-O8, and 43.9(4)° for O7’-Mn2-O8’ (Supplementary Fig. 2). By comparison with the structure of 1, the O-O bond distances in 2 are significantly different. Contrary to the shorter O-O bond length of 1 determined by the X-ray crystal structure, the crystallographically independent O-O bond distances for 2 are 1.415(2) (O1-O2), 1.412(3) (O7-O8) and 1.403(13) (O7’-O8’) Å, which are significantly longer than 1 and even longer than recently reported O–O bond distance of a mononuclear nonheme Mn(IV)-peroxo (1.379(3) Å) complex38. The average Mn–O distance of 1.876 Å is within the range of 1.841–1.901 Å for the monomeric side-on Mn(III)-peroxo complexes33. Moreover, this mean value is close to that of Mn(IV)-peroxo (1.878(2) Å) supported by tetradentate trianionic ligand38. Taken together, the spectroscopic and structural data unambiguously demonstrate that 2 is a high-spin (S = 3/2) Mn(IV)-peroxo complex binding the O22− unit in a side-on fashion (η2).
Computational correlation of the geometric and electronic structures of Fe(III)-superoxo and Mn(IV)-peroxo complexes
In order to elucidate the electronic structures of 1 and 2, DFT geometry optimizations were carried out on each complex starting from the atomic coordinates refined in both crystal structures and using its experimental spin state. For 1, a series of functionals employing varying amounts of Hartree-Fock exchange were used (BP86 [0%], TPSSh [10%], B3LYP [20%], BP86 + 25% HFX, and M06 [27%]), all with the TZVP basis set. The BP86 functional with 25% Hartree-Fock exchange gives an optimized structure most consistent with the crystallographic data (Supplementary Table 4 for geometric parameters). Note from Supplementary Table 4 that the O-O distance is most sensitive to functional and the experimental 1.32 Å distance, which reflects the amount of charge transfer from the metal to O2, is best reproduced by this hybrid functional (while M06 also gives a good O-O distance, its average Fe-O bond length is too long). For 2, the geometry optimization results using the BP86, TPSSh, B3LYP, and M06 functionals were compared, and the closest agreement with the experimental O-O bond length was obtained using B3LYP (Supplementary Table 5). Since it was necessary to use a BP86 + 25% HFX to reproduce the structure of 1, the Mössbauer parameters were recalculated using this functional (see Supplementary Fig. 15 for the calibration of experimental isomer shifts with calculated ρ at the Fe). The isomer shift obtained of δ = 0.11 mm s−1 is again consistent with the experimental value of 0.10 mm s−1.
Since the DFT-derived geometry-optimized structures for 1 and 2 give reasonable agreement with the experimental data, their associated electronic structures were evaluated. The unoccupied orbitals calculated for 1 with significant Fe d or O2 π* character are displayed in Fig. 4a (the unoccupied orbitals reflect the uncompensated electron density in the occupied orbitals). From Fig. 4a, the electronic structure of 1 lies between the limits of an S = 1 Fe(IV)-peroxo and an S = 3/2 Fe(III) antiferomagnetically coupled to an S = 1/2 superoxo species. The Mülliken population analysis of the unoccupied orbitals shows 78% O2 π*σ character (where π*σ is the in-[Fe-O2]-plane π* orbital on O2; π*v is out-of-plane) in the α-unoccupied dxz orbital and 15% O2 π*σ character in the β-unoccupied dxz orbital. From the Fe(IV)-peroxo limit, this is consistent with significant delocalization of an O22− electron onto the Fe and is thus closer to the Fe(III)-superoxo limit (Fig. 4b). The low energy unoccupied π*σ character on the superoxo is consistent with its H-atom abstraction reactivity. Similarly, for the calculated electronic structure of 2, starting from the Mn(IV)- peroxo limit, the unoccupied orbitals (Supplementary Fig. 16) show delocalization of αand β O22− π*σ electrons (47% and 21%, respectively) into the unoccupied Mn dxz orbital, in addition to 10% delocalization of the β O22− π*v electron into the β-unoccupied dxy orbital (which is occupied in 1) (Fig. 4c). This gives a total delocalization of 78% of a O22− π* electron onto the Mn, less than the delocalization onto Fe in 1 (93%), which places 2 closer to the Mn(IV)-peroxo limit, consistent with the longer O-O bond length in 2.
To understand the differences between the geometric and electronic structures of 1 and 2, an extra electron was added to each complex and the ionization energy of these hypothetical M(III)(TAML)-peroxo complexes was calculated, using the possible initial spin states (S = 3/2 for FeIII, S = 1 and 2 for MnIII) and the final spin states observed experimentally for 1 (S = 1) and 2 (S = 3/2). This evaluates whether there is a difference in the reduction potential between these metal ions in this ligand set. The results of these calculations are summarized in Supplementary Table 6, along with those of parallel calculations with a redox-innocent Cl− ligand in place of the peroxide. Relative to the iron complex, ionization of an electron from a hypothetical S = 1 Mn(III)-O22− is easier by 0.42 eV (1.23 eV with the Cl− ligand), while ionization of an electron from an S = 2 Mn(III)-O22− is approximately isoenergetic. From Fig. 4c and Supplementary Fig. 16, the electronic structure of 2 has significant S = 1 Mn(III) character. These calculations indicate that the reduction potential of the metal center in the MnO2 complex is lower than that of the metal center in the FeO2 complex, consistent with increased electron density donation from the peroxide in 1 that results in significant Fe(III)-superoxo character relative to 2.
In summary, we have reported the first synthetic mononuclear nonheme iron(III)-superoxo complex and a manganese(IV)-peroxo complex bearing a common supporting ligand. The crystal structures of those intermediates determined via single crystal X-ray crystallography demonstrate unambiguously that the O2 units in the iron(III)-superoxo and manganese(IV)-peroxo complexes are bound to the iron and manganese ions in a side-on fashion. The difference in electron distribution between the metal and the O2 reflects the higher reduction potential of the Fe(III). The nonheme iron(III)-superoxo complex undergoes both electrophilic and nucleophilic oxidation reactions. This model study suggests that both reactivities might be important in nonheme iron enzymecatalysis, which remains to be evaluated.
Methods
See experimental section in supplementary information for detailed experimental conditions and procedures, spectroscopic and kinetics analyses, and computational calculations.
Supplementary Material
Acknowledgments
The research was supported by NRF of Korea through the CRI (NRF-2012R1A3A2048842 to W.N.) and GRL (NRF-2010-00353 to W.N.) Programs. The publication was partially supported by National Institutes of Health (NIH) Grant Number 5 P41 RR001209 (E.I.S.).
Footnotes
Author contributions
W.N. and E.I.S. conceived and designed the experiments; S.H., K.D.S., J.P., and E.K. performed the experiments; S.H., K.D.S., and M.A.S. analysed the data; W.N., E.I.S., S.H., and K.D.S. co-wrote the paper.
Additional information
Supplementary information and chemical compound information accompany this paper at www.nature.com/naturecommunications.
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