Table 3. Multivariate analyses.
Analysis of Bray-Curtis (relative abundance) and Jaccard (presence-absence) dissimilarities among all samples (group based) and the R. globostellata, Callyspongia sp., and seawater specific subsets (habitat based). Results represent the three groups and ordination ellipses from Figs. 3A–3C and seawater with the number of 97%-OTUs available in each individual dataset for the betadisper/permutest (Dispersion) and adonis (PERMANOVA) analyses (1,000 permutations each). Attached are the nMDS stress values of the multivariate ordination from Figs. 3A–3C and seawater (seawater ordination not shown). Significant differences in bold.
Data matrix | Sample groups | Dispersion | PERMANOVA | ||||||
---|---|---|---|---|---|---|---|---|---|
OTUs | df | F value | p value | F value | R2 | p value | nMDS stress | ||
Relative abundance | Sponges & water (groups) | 2,247 | 21 | 1.75 | >0.99 | 22.09 | 0.81 | <0.001 | 0.08 |
R. globostellata (habitat) | 341 | 4 | 0.01 | >0.99 | 1.80 | 0.31 | 0.1 | 0.01 | |
Callyspongia sp. (habitat) | 673 | 5 | 0.29 | >0.99 | 2.85 | 0.36 | <0.001 | <0.01 | |
Seawater (habitat) | 1,537 | 3 | 5.23 | 0.02 | 6.28 | 0.68 | 0.008 | <0.01 | |
Presence-absence | Sponges & water (groups) | 2,247 | 21 | 31.38 | 0.02 | 6.15 | 0.54 | <0.001 | 0.09 |
R. globostellata (habitat) | 341 | 4 | 0.50 | >0.99 | 1.64 | 0.290 | 0.1 | <0.01 | |
Callyspongia sp. (habitat) | 673 | 5 | 0.26 | >0.99 | 1.85 | 0.270 | <0.001 | <0.01 | |
Seawater (habitat) | 1537 | 3 | 0.62 | >0.99 | 2.4 | 0.45 | 0.1 | <0.01 |