Table 2. Autolysis scores^a for fresh, refrigerated, room temperature, and frozen tissue samples taken at 0, 4, 24, and 48 h.^b.

Samples	Gill arch	Skin	Skeletal muscle	Kidney	Liver/pancreas	Spleen	Gonads	Heart	GI	Brain
0 h Fresh	–	–	–	Minimal	Minimal	–	–	–	–	–
4 h Ref.	Minimal	Minimal	Minimal	Minimal	Minimal	Minimal	Minimal	Minimal	Minimal	Minimal
4 h Z	Mild	Minimal	Mild to moderate	Mild	Minimal	Minimal	Minimal	Minimal	Mild	Mild
4 h RT	Mild to moderate	Minimal	Minimal	Mild	Minimal	Minimal	Minimal	Minimal	Mild	Mild
24 h Ref.	Moderate	Minimal	Minimal	Mild	Mild	Mild	Mild	Mild	Mild	Mild
24 h Z	Severe	Moderate	Mild to moderate	Mild to moderate	Mild to moderate	Mild to moderate	Mild	Mild	Mild to moderate	Mild to moderate
24 h RT	Severe	Moderate	Mild to moderate	Moderate	Moderate	Moderate	Moderate	Moderate	Moderate to severe	Moderate to severe
48 h Ref.	Moderate to severe	Moderate	Mild to moderate	Moderate	Moderate	Mild to moderate	Mild to moderate	Mild to moderate	Severe	Mild to moderate
48 h RT	Severe	Severe	Moderate	Severe	Severe	Severe	Severe	Severe	Severe	Severe
48 h Z	Severe	Severe	Mild to moderate	Moderate	Moderate to severe	Moderate	Moderate	Mild to moderate	Mild to moderate	Mild to moderate

Notes.

^aMinimal (<5%); Mild (5–10%); Moderate (10–50%); Severe (>50%).

^bRef., refrigerated; Z, frozen; RT, room temperature; h, hour(s); GI, gastro-intestinal.