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. Author manuscript; available in PMC: 2017 Jun 2.

Published in final edited form as: Neuroscience. 2016 Mar 19;324:446–468. doi: 10.1016/j.neuroscience.2016.03.036

Schematic drawings of different brain regions showing the pattern of location of retrogradely-labeled neurons within the nuclei from neonatal rats injected with rhodamine beads into cNTS. The schematic drawings are taken from Paxinos and Watson (1997). A: A5 region; 106 neurons with rhodamine-loaded soma were counted in 8 slices between Bregma −9.00 and −11.28 mm; of these, 72% were localized to the caudal portion of the A5 region; B: SubC: subcoeruleus nucleus alpha portion (SubCA), dorsal portion (SubCD) and ventral portion (SubCV); 103 neurons with rhodamine-loaded soma were counted in 4 slices between Bregma −8.88 and −9.96 mm; of these, 12% were localized to the SubCA region, 39% to the SubCD region, and 49% to the SubCV region (red circle); C: LC: locus coeruleus; 58 neurons with rhodamine-loaded soma were counted in 4 slices between Bregma −8.80 and −10.04 mm; of these, 51% were localized to the dorsal region of the LC (red circle); D: PAG: periaqueductal gray dorsomedial portion (dmPAG), dorsolateral portion (dlPAG), lateral portion (lPAG) and ventrolateral portion (vlPAG); 48 neurons with rhodamine-loaded soma were counted in 6 slices between Bregma −7.30 and −8.80 mm; of these, 15% were localized to the dmPAG region, 32% in the dlPAG region, 21% in the lPAG region and 32% in the vlPAG region (all areas defined by the dashed red line); E: RTN: retrotrapezoid nucleus; 30 neurons with rhodamine-loaded soma were counted in 3 slices between Bregma −10.44 and −10.80 mm; of these, 100% were localized to the caudal region and central area of the RTN (red circle); F: KF: Kölliker-Fuse; 41 neurons with rhodamine-loaded soma were counted in 2 slices between Bregma −8.72 and −8.80 mm; of these, 83% were localized to the ventral portion of the KF (red circle); G: Pre-Bötz: Pre-Bötzinger complex; 64 neurons with rhodamine-loaded soma were counted in 2 slices between Bregma −12.30 and −12.84 mm; of these, 72% were localized to the ventral portion of the Pre-Bötz (red circle); H: Bötz: Bötzinger complex; 50 neurons with rhodamine-loaded soma were counted in 2 slices between Bregma −11.96 and −12.48 mm; of these, 72% were localized to the ventral portion of the Bötz (red circle); I: lHyp: lateral hypothalamus. 63 neurons with rhodamine-loaded soma were counted in 6 slices between Bregma −1.2 and −4.56 mm; of these, 77% were localized to the ventral portion of the lHyp (red circle);

Schematic drawings of different brain regions showing the pattern of location of retrogradely-labeled neurons within the nuclei from neonatal rats injected with rhodamine beads into cNTS. The schematic drawings are taken from Paxinos and Watson (1997). A: A5 region; 106 neurons with rhodamine-loaded soma were counted in 8 slices between Bregma −9.00 and −11.28 mm; of these, 72% were localized to the caudal portion of the A5 region; B: SubC: subcoeruleus nucleus alpha portion (SubCA), dorsal portion (SubCD) and ventral portion (SubCV); 103 neurons with rhodamine-loaded soma were counted in 4 slices between Bregma −8.88 and −9.96 mm; of these, 12% were localized to the SubCA region, 39% to the SubCD region, and 49% to the SubCV region (red circle); C: LC: locus coeruleus; 58 neurons with rhodamine-loaded soma were counted in 4 slices between Bregma −8.80 and −10.04 mm; of these, 51% were localized to the dorsal region of the LC (red circle); D: PAG: periaqueductal gray dorsomedial portion (dmPAG), dorsolateral portion (dlPAG), lateral portion (lPAG) and ventrolateral portion (vlPAG); 48 neurons with rhodamine-loaded soma were counted in 6 slices between Bregma −7.30 and −8.80 mm; of these, 15% were localized to the dmPAG region, 32% in the dlPAG region, 21% in the lPAG region and 32% in the vlPAG region (all areas defined by the dashed red line); E: RTN: retrotrapezoid nucleus; 30 neurons with rhodamine-loaded soma were counted in 3 slices between Bregma −10.44 and −10.80 mm; of these, 100% were localized to the caudal region and central area of the RTN (red circle); F: KF: Kölliker-Fuse; 41 neurons with rhodamine-loaded soma were counted in 2 slices between Bregma −8.72 and −8.80 mm; of these, 83% were localized to the ventral portion of the KF (red circle); G: Pre-Bötz: Pre-Bötzinger complex; 64 neurons with rhodamine-loaded soma were counted in 2 slices between Bregma −12.30 and −12.84 mm; of these, 72% were localized to the ventral portion of the Pre-Bötz (red circle); H: Bötz: Bötzinger complex; 50 neurons with rhodamine-loaded soma were counted in 2 slices between Bregma −11.96 and −12.48 mm; of these, 72% were localized to the ventral portion of the Bötz (red circle); I: lHyp: lateral hypothalamus. 63 neurons with rhodamine-loaded soma were counted in 6 slices between Bregma −1.2 and −4.56 mm; of these, 77% were localized to the ventral portion of the lHyp (red circle);