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Fluorescent images of ectopic bone showing Tie2-lineage cells (tdTomato) overlaid with tartrate-resistant acid phosphatase/TRAP activity stain (ELF97, green) to label osteoclasts (A, zoom in B). No overlap was seen between Tie2-lineage cells with an alkaline phosphatase/AP activity stain (ELF97) to label osteoblasts (C, zoom in D). Scale bar = 50 μm.
