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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Allergy. 2016 Mar 6;71(5):621–628. doi: 10.1111/all.12812

Figure 2.

Figure 2

Fusion protein-selected antibodies recognize a 30 kDa Ae. aegypti salivary protein.

A. Mouse antibodies. Ae. aegypti saliva proteins were separated by SDS-PAGE and immunoblotted with mouse anti-saliva serum (lane 1), fusion protein-selected mouse antibodies (lane 2), or PBST (lane 3), followed by incubation with peroxidase-conjugated goat anti-mouse IgG.

B. Human antibodies. Ae. aegypti salivary proteins were separated by SDS-PAGE and immunoblotted with a pooled human mosquito-allergic serum (lane 1), fusion protein-selected human antibodies (lane 2), or PBST (lane 3), followed by incubation with mAb anti-human IgE.