The following information is missing from the funding section: This work was supported by grants from Fondo Clemente Estable-ANII (FCE_6353) to AT and (FCE_6363) to GP, CSIC-Grupos (Uruguay) to HR and RR. BSC was supported by a postdoctoral fellowship by ANII-Uruguay. NR was partially supported by a fellowship from Sistema Nacional de Becas-ANII.
In Panel B of Fig 1, the x-axis is missing. Please see the corrected Fig 1 here.
Fig 1. ANG II-mediatedO2.-production is inhibited by NO2-AA.
(A) Dehydroethydium (DHE) oxidation as an index of O2.- production in ANG II-stimulated HK-2 cells (1x106 cells) was determined by HPLC with fluorescence detection (λem = 595 nm, λexc = 510 nm). Cells were preincubated 30 min with vehicle, 10 μM NO2-AA or 10 μM AA and then exposed to DHE in simultaneous with 0.1 μM ANG II for 3 h. Quantitative analysis, expressed as the mean fold change than control condition without ANG II stimulation ± SEM, n = 3 of 2-OH-Et+ (B) and Et+ (C) are shown. *, # correspond to significant data relative to control and ANG II-treated cells, respectively (p<0.05).
Reference
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