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. Author manuscript; available in PMC: 2016 Apr 27.
Published in final edited form as: Placenta. 2014 Jun 6;35(8):645–654. doi: 10.1016/j.placenta.2014.05.005

Fig. 1.

Fig. 1

Overview of human placental tissue banking and collection methods. Human placenta from first and third trimester pregnancies were processed immediately after delivery. Samples were washed in PBS and cut into small pieces (0.1 cm3). The small samples were then placed into sterile 1.5 mL microfuge tubes and either snap-frozen in liquid nitrogen, placed into tubes containing 1 ml of RNAlater™, or DNAgard®. Samples that were snap-frozen were stored in a −80 °C freezer. Samples in DNAgard were stored at room temperature until the time of DNA extraction (within 1 month). Samples in RNAlater were immediately placed at 4 °C Larger samples, “chunks” (1 cm3) were processed with RNAlater only.