Table 3.
Antibodies and Staining Conditions for Immunocytochemistry
| Antibody | Vendor, clone or product#, species | Fixative | Dilution | Block | Secondary Antibody |
|---|---|---|---|---|---|
| PECAM-1 | Thermo Sci (Labvision), RB-10333, Rb polyclonal | MeOH only | 1:25 | 10% PBSG | Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-11034) |
| Glut-1 | Thermo Sci (Labvision), SPM498, Mouse IgG2a | MeOH | 1:100–1:500 | 10–40% PBSG | Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-11001) |
| VE-cadherin | Santa Cruz, BV9, Ms IgG2a | MeOH | 1:25 | 10–40% PBSG | Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-11001) Goat anti-Mouse IgG2a Secondary Antibody, Alexa Fluor® 594 conjugate (Life A-21135) |
| Santa Cruz, F-8, IgG1 | MeOH | 1:100 | 10–40% PBSG | Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-11001) | |
| Occludin | Invitrogen, OC-3F10, IgG1 | MeOH | 1:200 | 10–40% PBSG | Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-11001) |
| ZO-1 | Invitrogen, 40–2200, Rb polyclonal | MeOH | 1:100 | 10-% PBSG | Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-11001) |
| Claudin-5 | Invitrogen, 4C3C2, IgG1 | MeOH | 1:50 | 10–40% PBSG | Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-11001) |
| P-gp | Thermo Scientific (Neomarkers), p170 (F4), Mouse IgG1 | MeOH | 1:25 | 10% PBSG | Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-11001) |
| BCRP | Millipore, MAB4155 clone 5D3, Mouse IgG2b-kappa | 4% PFA only | 1:50 | 10% PBSG | Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-11001) |
| MRP1 | Millipore, MAB4100 clone QCRL1, Mouse IgG1 | MeOH | 1:25 | 40% PBSG | Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-11001) |
| Nestin | Millipore, 10C2, Ms IgG1 | MeOH | 1:500 | 40% PBSG | Goat anti-Mouse IgG1 Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-21121) |
| Antibody | Vendor, clone or product #, species | Fixative | Dilution | Block | Secondary Antibody |
|---|---|---|---|---|---|
| PECAM-1 | Thermo Sci (Labvision), RB-10333, Rb polyclonal | MeOH only | 1:25 | 10% PBSG | Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-11034) |
| Glut-1 | Thermo Sci (Labvision), SPM498, Mouse IgG2a | MeOH | 1:100–1:500 | 10–40% PBSG | Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-11001) Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-11001) |
| VE-cadherin | Santa Cruz, BV9, Ms IgG2a | MeOH | 1:25 | 10–40% PBSG | Goat anti-Mouse IgG2a Secondary Antibody, Alexa Fluor® 594 conjugate (Life A-21135) |
| Santa Cruz, F-8, IgG1 | MeOH | 1:100 | 10–40% PBSG | Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-11001) | |
| Occludin | Invitrogen, OC-3F10, IgG1 | MeOH | 1:200 | 10–40% PBSG | Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-11001) |
| ZO-1 | Invitrogen, 40–2200, Rb polyclonal | MeOH | 1:100 | 10-% PBSG | Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-11034) |
| Claudin-5 | Invitrogen, 4C3C2, IgG1 | MeOH | 1:50 | 10–40% PBSG | Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-11001) |
| P-gp | Thermo Scientific (Neomarkers), p170 (F4), Mouse IgG1 | MeOH | 1:25 | 10% PBSG | Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-11001) |
| BCRP | Millipore, MAB4155 clone 5D3, Mouse IgG2b | 4% PFA only | 1:50 | 10% PBSG | Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-11001) |
| MRP1 | Millipore, MAB4100 clone QCRL1, Mouse IgG1 | MeOH | 1:25 | 40% PBSG | Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-11001) |
| Nestin | Millipore, 10C2, Ms IgG1 | MeOH | 1:500 | 40% PBSG | Goat anti-Mouse IgG1 Secondary Antibody, Alexa Fluor® 488 conjugate (Life A-21121) |