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. Author manuscript; available in PMC: 2016 Apr 28.
Published in final edited form as: Electrophoresis. 2014 Jun 16;35(24):3418–3429. doi: 10.1002/elps.201400017

Figure 5.

Figure 5

Distributions of identified phosphopeptides from HeLa cell lysate by combinatorial ERLIC–SCX fractionation. The number and percentage of nonredundant identified mono- and multiphosphorylated peptides by ERLIC-SCX-TiO2 (A). (B) The number and percentage of nonredundant identified mono- and multiphosphorylated peptides by ERLIC. (C) The number and percentage of nonredundant identified mono- and multiphosphorylated peptides from further separation of flow-through fractions of ERLIC by SCX. (D) Distribution of identified mono- and multiphosphorylated peptides in each SCX fraction. (E) Venn diagram for nonredundant identified phosphopeptides in both methods. Different colors represent the number of phosphate groups (1p–4,5p) carried by each phosphopeptide. Reprinted with permission from Ref [100]. Copyright American Chemical Society 2012.