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. 1992 Mar 1;89(5):1621–1625. doi: 10.1073/pnas.89.5.1621

Regulation of Cl- channels in normal and cystic fibrosis airway epithelial cells by extracellular ATP.

M J Stutts 1, T C Chinet 1, S J Mason 1, J M Fullton 1, L L Clarke 1, R C Boucher 1
PMCID: PMC48504  PMID: 1371880

Abstract

The rate of Cl- secretion by human airway epithelium is determined, in part, by apical cell membrane Cl- conductance. In cystic fibrosis airway epithelia, defective regulation of Cl- conductance decreases the capability to secrete Cl-. Here we report that extracytosolic ATP in the luminal bath of cultured human airway epithelia increased transepithelial Cl- secretion and apical membrane Cl- permeability. Single-channel studies in excised membrane patches revealed that ATP increased the open probability of outward rectifying Cl- channels. The latter effect occurs through a receptor mechanism that requires no identified soluble second messengers and is insensitive to probes of G protein function. These results demonstrate a mode of regulation of anion channels by binding ATP at the extracellular surface. Regulation of Cl- conductance by external ATP is preserved in cystic fibrosis airway epithelia.

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