Fig. 2.

Thermostabilised ChAdOx1-GnGc elicits functional antibody. The titre of RVFV neutralising antibody elicited by vaccination with thermostabilised ChAdOx1-GnGc following 6 months storage at the indicated temperatures is shown. Briefly, sera were heat-inactivated at 56 °C for 30 min and serially diluted in quadruplicate in buffer before incubation with 100TCID₅₀ of RVFV MP-12 strain for 60 min at 37 °C. This serum-virus mixture was then transferred onto confluent Vero cell monolayers, incubated at 37 °C and 5% CO₂ for 72 h, fixed and stained, and endpoint titres calculated by the Spearman-Karber method as described [15]. Data represent mean ± standard deviations. A two-tailed p-value representing a between-group comparison by the Kruskal−Wallis test is shown. The 55 °C and unvaccinated (control) groups had no detectable response and are excluded from this analysis.