Skip to main content
. Author manuscript; available in PMC: 2016 May 2.
Published in final edited form as: Neuron. 2008 Jun 12;58(5):763–774. doi: 10.1016/j.neuron.2008.03.030

Figure 4. VFO and Spikelets, but Not Gamma Rhythms, Survive in Calcium-Free Extracellular Medium.

Figure 4

(A) Blockade of phasic transmitter release via removal of calcium ions from the extracellular bathing solution (aCSF) abolished gamma frequency population oscillations but did not affect the VFO. Blockade of the main transmembrane calcium channels in Purkinje cells with ω-agatoxin (ω-ATX, 100 nM) had no effect on calcium-free VFO. However, blockade of fast sodium channels with tetrodotoxin (TTX, 100 nM) abolished population VFO rhythms. Bath application of the broad-spectrum gap junction blocker carbenoxolone (CBX, 0.1 mM) also abolished population rhythms. Scale bars: 0.2 mV, 50 ms.

(B) Population VFO was accompanied by phase-locked trains of spikelets in individual Purkinje cells. Traces show concurrently recorded somatic Purkinje cell response and local field potential VFO. Graph shows tight temporal correlation between spikelet peak and the trough of the corresponding field potential VFO, cf. Figure 3C (n = 200 spikelets from n = 5 Purkinje cells). Scale bars: 0.2 mV (field), 2 mV (intracellular), 10 ms.