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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Steroids. 2016 Apr 13;110:49–61. doi: 10.1016/j.steroids.2016.04.002

Figure 1.

Figure 1

The effect of Vitamin D compounds on the proliferation of HaCaT keratinocytes treated with hydrogen peroxide. HaCaT keratinocytes were treated with serial dilutions (10−12–10−6 M) of 1,25(OH)2D3 (A), 20(OH)D3 (B), 21(OH)pD (C) or calcipotriol (D), or were treated with serial dilutions of H2O2 (0.0256–5 mM) alone or in combination with 10 nM 1,25(OH)2D3 (E), 20(OH)D3 (F), 21(OH)pD (G) or calcipotriol (H) for 24 h (according to Scheme 1A). Data are shown as mean from three independent experiments ± S.D. Statistical significance was estimated using One–Way ANOVA and presented as *p < 0.05, **p < 0.005, ***p < 0.0005 versus control. Please note that the same curve showing the response to H2O2 alone was included into panels E – H in order to underline the effects of co-incubation with vitamin D analogues.