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. 2016 May 3;6:25343. doi: 10.1038/srep25343

Induction of P-glycoprotein expression and activity by Aconitum alkaloids: Implication for clinical drug–drug interactions

Jinjun Wu 1, Na Lin 1,3, Fangyuan Li 1, Guiyu Zhang 1, Shugui He 1, Yuanfeng Zhu 1, Rilan Ou 1, Na Li 2, Shuqiang Liu 1, Lizhi Feng 1, Liang Liu 2, Zhongqiu Liu 1,2, Linlin Lu 1,2,a
PMCID: PMC4853792  PMID: 27139035

Abstract

The Aconitum species, which mainly contain bioactive Aconitum alkaloids, are frequently administered concomitantly with other herbal medicines or chemical drugs in clinics. The potential risk of drug–drug interactions (DDIs) arising from co-administration of Aconitum alkaloids and other drugs against specific targets such as P-glycoprotein (P-gp) must be evaluated. This study focused on the effects of three representative Aconitum alkaloids: aconitine (AC), benzoylaconine (BAC), and aconine, on the expression and activity of P-gp. We observed that Aconitum alkaloids increased P-gp expression in LS174T and Caco-2 cells in the order AC > BAC > aconine. Nuclear receptors were involved in the induction of P-gp. AC and BAC increased the P-gp transport activity. Strikingly, intracellular ATP levels and mitochondrial mass also increased. Furthermore, exposure to AC decreased the toxicity of vincristine and doxorubicin towards the cells. In vivo, AC significantly up-regulated the P-gp protein levels in the jejunum, ileum, and colon of FVB mice, and protected them against acute AC toxicity. Taken together, the findings of our in vitro and in vivo experiments indicate that AC can induce P-gp expression, and that co-administration of AC with P-gp substrate drugs may cause DDIs. Our findings have important implications for Aconitum therapy in clinics.

Aconitine (AC) is one of the main bioactive Aconitum alkaloids present in the Aconitum species (Ranunculaceae family), and is widely used in China and other Asian countries to treat rheumatoid arthritis, cardiovascular diseases, and tumors1,2. Unfortunately, AC is also the most toxic diester alkaloid among Aconitum alkaloids. It can stimulate Na+ channels and is therefore a strong neurotoxin and painkiller3,4,5,6. For this reason, application of Aconitum alkaloids is restricted in clinics. When heated or hydrolyzed by the intestinal hydrolase, AC is easily converted into benzoylaconine (BAC) or aconine (Supplementary Fig. S1)7,8. Hydrolysis of AC decreases its toxicity by over 100-fold7,9,10.

P-glycoprotein (P-gp, MDR1) is an important protein located on the apical membrane of mature epithelial cells of different organs11,12. It functions as an efflux pump and plays a crucial role in protecting the human body by pumping external chemicals out of cells13. However, P-gp expression and activity are frequently changed by its own substrates, potentially affecting its pharmacokinetics, bioavailability, toxicity, and therapeutic response, which is recognized by authorities as one of the most important causes of drug-drug interactions (DDIs) among P-gp substrates14,15,16. Thus, investigating the effects of substrate drugs on P-gp can provide useful information for clinical use of P-gp substrate drugs.

Nuclear receptors (NRs) are ligand-inducible transcription factors that specifically regulate the expression of phase I and phase II drug-metabolizing enzymes, as well as xenobiotic transporters16,17,18. Among the NRs, the pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are considered key transcriptional regulators of P-gp19. Several studies have reported the various agonists of PXR and CAR20,21,22,23,24,25, and extensive reviews have been written on the regulation of xenobiotic transporters by PXR and CAR16,19.

Previous studies have demonstrated that P-gp is the main ABC transporter involved in AC efflux26,27,28. Our previous studies also confirmed that P-gp mediates the transport of Aconitum alkaloids, and the effect of P-gp on transport follows the trend AC > BAC > aconine29. However, little is known about the effects of the three alkaloids on P-gp. Whether AC, BAC, or aconine can modulate P-gp via NRs, specifically via PXR or/and CAR, has never been studied. More importantly, Aconitum species are frequently used in combination with other herbal medicines, including Ginseng radix, Zingiberis rhizoma, Liquiritiae radix, Rhei radix, and Paeoniae radix alba, for toxicity reduction30. Some of the component herbs and ingredients have been confirmed to alter P-gp expression and activity. For example, Liquiritiae radix and its main bioactive compounds, including glycyrrhizin, glycyrrhetinic acid, and liquiritin, can significantly increase P-gp expression and activity31,32,33. Besides, Aconitum species are likely to be administered concomitantly with other chemical drugs that are substrates of P-gp to treat complex diseases. For example, digoxin and verapamil are substrates of P-gp, and are usually co-administered with Aconitum species to treat cardiovascular diseases34. Several anti-tumor drugs, such as paclitaxel, doxorubicin, and vincristine, are also substrates of P-gp35; these drugs are usually co-administrated to achieve maximum treatment efficacy against cancer. Any effect of the Aconitum alkaloids on P-gp expression and/or activity might cause DDIs, thereby resulting in undesirable variation in the plasma concentrations of co-administered substrate drugs, with treatment failure or toxicologically unsafe consequences. Therefore, a thorough assessment of DDI risk with co-administration of Aconitum alkaloids and P-gp substrates drugs is essential and urgent.

For this purpose, we first evaluated the effects of AC, BAC, and aconine on the expression of P-gp in LS174T and Caco-2 cells. These two cell lines are suitable in vitro models to study P-gp induction, localization, and function by xenobiotic drugs21,36,37,38,39. We also confirmed the regulatory effects of the tested drugs in FVB mice in vivo. Second, we investigated whether the three Aconitum alkaloids can modulate P-gp via NRs, specifically via PXR or/and CAR. Third, we explored the tested drugs on the function of P-gp in both cell lines. Fourth, we determined if changes in the expression and activity of P-gp can affect the cells against cytotoxicity produced by vincristine and doxorubicin; both drugs are used in cancer chemotherapy and as model P-gp substrates35. Finally, we investigated if pretreatment of AC can protect the FVB mice against acute AC toxicity. The results of this study can help predict potential risks of P-gp-related DDIs between Aconitum alkaloids and other co-administered drugs. Thus, our findings have important implications for the correct clinical application of Aconitum alkaloids.

Results

Effects of AC, BAC, and aconine on P-gp protein and mRNA levels

Protein levels of P-gp were evaluated by Western blot analysis. Compared with the control cells, P-gp protein levels in the tested groups gradually increased over 6 days of incubation with increasing dose in the order AC > BAC > aconine at both 50 μM and 100 μM concentration (Fig. 1A). AC also increased the P-gp protein levels in a time-dependent manner, and the increase was significantly higher than those in the BAC and aconine groups (Fig. 1B). A significant increase in P-gp levels (p < 0.001) was observed when rifampicin (20 μM), a model inducer of P-gp, was used to treat the LS174T cells (Fig. 1C). Treatment of Caco-2 cells with AC, BAC, and aconine (50 μM, 6 days) significantly increased the P-gp levels and the effect followed the trend AC > BAC > aconine (Fig. 1D). Treatment with 0.4 and 0.6 mg/kg AC for 14 days also significantly increased the P-gp levels in the jejunum, ileum, and colon of FVB mice (Fig. 1E). The relative MDR1 mRNA levels were further analyzed in both cell lines by real-time PCR. AC treatment (50 μM, 6 days) significantly increased the MDR1 mRNA levels in LS174T (Fig. 1F) and Caco-2 (Fig. 1G) cells in the order AC > BAC > aconine, which was consistent with our results showing increased P-gp protein levels under these conditions. As shown in Fig. 2, our immunostaining data for P-gp in LS174T and Caco-2 cells were also consistent with data obtained from Western blot analysis showing changes in total P-gp protein levels.

Figure 1. Effects of AC, BAC, and aconine on P-gp protein and mRNA levels.

Figure 1

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P-gp protein levels were detected by Western blot analysis. mRNA levels were detected by real-time PCR analysis. (A) Dose-dependent effects on P-gp protein levels in LS174T total lysates after treatment with the tested drugs (5–100 μM) or the vehicle (control) for 6 days. (B) Time-dependent effects on P-gp protein levels in LS174T total lysates after treatment with the tested drugs (50 μM) or the vehicle (control) for 2–8 days. (C) LS174T cells were treated with rifampicin (Rif, 20 μM) for 2–8 days as the positive control for P-gp induction. (D) P-gp protein levels in Caco-2 cells after treatment with the tested drugs (50 μM) or the vehicle (control) for 6 days. (E) P-gp protein levels in FVB mice after treatment with AC (0.4/0.6 mg/kg) or the vehicle (control) for 14 days. MDR1 mRNA levels in LS174T (F) and Caco-2 (G) cells after treatment with the tested drugs (50 μM) or the vehicle (control) for 6 days. Densitometry results were related to β-actin and presented as the percentage of controls. GAPDH was used as the housekeeping gene for cells. Data represent mean ± SD (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the control group; #p < 0.05 compared with the AC group; Δp < 0.05 compared with the BAC group.

Figure 2.

Figure 2

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Effects of AC, BAC, and aconine on P-gp immunofluorescence in LS174T (A) and Caco-2 (B) cells by confocal microscopy. Cells were pretreated with AC, BAC, or aconine (50 μM) or with the vehicle (control) for 6 days. The scale bar represents 25 μm.

NRs mediate P-gp induction via AC/BAC/aconine

Ketoconazole (a non-specific NR inhibitor) did not modify P-gp expression in LS174T cells. However, when incorporated with AC, BAC, or aconine, ketoconazole significantly prevented increase in P-gp protein levels induced by the three alkaloids. The striking increase in P-gp levels induced by rifampicin was also significantly reversed by combined incubation with ketoconazole (Fig. 3A). The same treatment also increased PXR protein levels in LS174T cells, with the effect following the order AC > BAC > aconine. The baseline CAR levels were too low to be detected in LS174T cells in all untreated and treated groups. RXRα remained unchanged after the treatment (Fig. 3B). Ketoconazole also significantly reversed P-gp induction by the three alkaloids in Caco-2 cells (Fig. 3C). Both PXR and CAR levels increased in the order AC > BAC > aconine. No marked induction was observed in the RXRα levels (Fig. 3D). The same trend of increase in PXR mRNA levels was observed in LS174T cells (Fig. 4A). Increase in PXR and CAR mRNA levels was found in Caco-2 cells (Fig. 4B), which was consistent with protein variations. Correlations between MDR1 mRNA levels and PXR or CAR protein levels were analyzed. The MDR1 mRNA levels was closely related to the PXR protein levels in LS174T cells (R2 = 0.961, p < 0.05; Fig. 5A). Strong correlations were also observed between MDR1 mRNA levels and PXR protein levels (R2 = 0.951, p < 0.05) and CAR protein levels (R2 = 0.954, p < 0.05) in Caco-2 cells (Fig. 5B).

Figure 3. Mediation of NRs in P-gp protein up-regulation by AC, BAC, and aconine.

Figure 3

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Effects of KTZ on AC-, BAC-, or aconine-mediated P-gp induction in LS174T (A) and Caco-2 (C) cells. Ketoconazole (KTZ) (50 μM) was used as a non-specific NR inhibitor. Cells were exposed to the vehicle (control), KTZ (50 μM), AC/BAC/aconine (50 μM), or AC/BAC/aconine (50 μM) +KTZ (50 μM) for 6 days. Rifampicin (Rif, 20 μM) was used as positive control in LS174T cells. (B) Effects of AC, BAC, and aconine on PXR and RXRα protein levels in LS174T cells. (D) Effects of AC, BAC, and aconine on PXR, CAR, and RXRα protein expression levels in Caco-2 cells. Proteins were detected by Western blot analysis and presented as the percentage of control. Data represent mean ± SD (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the control group or the corresponding groups; #p < 0.05 compared with the AC group; Δp < 0.05 compared with the BAC group.

Figure 4. Effects of AC, BAC, and aconine on the mRNA levels of NRs.

Figure 4

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Cells were treated with AC, BAC or aconine (50 μM) or vehicle (control) for 6 days. mRNA levels were detected by real-time PCR analysis. (A) Effects of AC, BAC, and aconine on PXR, PPARγ, GR, AhR, VDR and RXRα mRNA expression levels in LS174T cells. (B) Effects of AC, BAC, and aconine on PXR, CAR, PPARγ, GR, AhR, VDR and RXRα mRNA levels in Caco-2 cells. GAPDH was used as housekeeping genes for cells. Data represent mean ± SD (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the control group; #p < 0.05 compared with the AC group; Δp < 0.05 compared with the BAC group.

Figure 5.

Figure 5

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Pairwise correlation between MDR1 mRNA levels and PXR or CAR protein levels in LS174T (A) and Caco-2 (B) cells (linear regression analysis, Pearson’s correlation).

Effects of AC, BAC, and aconine on P-gp transport activity in LS174T and Caco-2 cells

Intracellular accumulation of rhodamine 123 was inversely correlated with P-gp extrusion activity. Treatment of LS174T cells with AC, BAC, and aconine (50 μM, 6 days) decreased rhodamine 123 accumulation by 22% (p < 0.01), 14% (p < 0.05), and 5% (p > 0.05), respectively, compared to control cells (Fig. 6A). In Caco-2 cells, the same treatment also resulted in decreased rhodamine 123 accumulation by 24% (p < 0.001), 15% (p < 0.01), and 2% (p > 0.05), respectively (Fig. 6B). Verapamil (50 μM and 100 μM in LS174T and Caco-2 cells, respectively) was used as a positive drug for P-gp inhibition. In both cell lines, the decrease in rhodamine 123 accumulation was reversed by verapamil, which indicated that the decrease was P-gp-dependent.

Figure 6. Effects of AC, BAC, and aconine on P-gp function.

Figure 6

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Cells were treated with AC, BAC, or aconine (50 μM) or with the vehicle (control) for 6 days. Rhodamine 123 (Rh123) accumulation in LS174T (A) and Caco-2 cells (B) was measured by flow cytometry, recorded in histograms in the presence or absence of verapamil (Ver) (50 μM and 100 μM for LS174T and Caco-2 cells, respectively), and inversely correlated with P-gp transport activity. Data were expressed as the percentage of control and represent mean ± SD (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the control group; #p < 0.05 compared with the AC group; ■p < 0.05 compared with the BAC group; Δp < 0.05 compared with the aconine group.

Effects of AC, BAC, and aconine on ATP levels and mitochondrial mass in LS174T and Caco-2 cells

Compared with the control, LS174T cells exposed to AC, BAC, and aconine (50 μM, 6 days) demonstrated increased intracellular ATP levels by 21% (p < 0.01), 11% (p < 0.05) and −3% (p > 0.05), respectively (Fig. 7A). The increase in Caco-2 cells was 38% (p < 0.01), 16% (p < 0.05), and 1% (p > 0.05), respectively (Fig. 7B). Incubation of LS174T cells with the same treatment increased the mean fluorescence intensity of NAO by 21% (p < 0.001), 5% (p > 0.05), and 2% (p > 0.05), respectively, with respect to the control cells (Fig. 7C). In Caco-2 cells, the increase in mean fluorescence intensity was 18% (p < 0.001), 9% (p < 0.01), and 2% (p > 0.05), respectively (Fig. 7D).

Figure 7. Effects of AC, BAC, and aconine on intracellular ATP levels and intracellular mitochondrial mass.

Figure 7

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Cells were treated with AC, BAC, or aconine (50 μM) or with the vehicle (control) for 6 days. ATP levels in LS174T (A) and Caco-2 cells (B) were measured with a firefly luciferase ATP assay kit. Fluorescence intensity of NAO in LS174T (C) and Caco-2 cells (D) was measured by flow cytometry and recorded in histograms. Data were expressed as the percentage of control and represent mean ± SD (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the control group; #p < 0.05 compared with the AC group; Δp < 0.05 compared with the BAC group.

Effects of AC, BAC, and aconine against vincristine and doxorubicin cytotoxicity

Table 1 shows the protective effect of AC/BAC/aconine (50 μM, 6 days) against vincristine and doxorubicin (two substrates of P-gp) cytotoxicity in both cell lines. In LS174T cells, the IC50 value related to vincristine cytotoxicity in the AC group was significantly higher (p < 0.05) than that in the control group. Similarly, incubation of Caco-2 cells with AC and BAC significantly (p < 0.01 and p < 0.05, respectively) increased the IC50 value related to vincristine cytotoxicity. Treatment of LS174T cells with AC also significantly (p < 0.01) increased the IC50 value related to doxorubicin cytotoxicity. Similarly, the IC50 value related to doxorubicin cytotoxicity was significantly higher (p < 0.01 or p < 0.05) in Caco-2 cells treated with AC and BAC compared to control cells. Verapamil and tariquidar were used as positive controls for P-gp inhibition. The results showed that the IC50 values related to vincristine and doxorubicin cytotoxicity were significantly lower (p < 0.001) in cells treated with verapamil and tariquidar compared to control cells.

Table 1. Effects of AC, BAC, and aconine on vincristine and doxorubicin-induced cytotoxicity.

Treatment LS174T IC50 (μM) Caco-2 IC50 (μM)
Vincristine (Control) 56.81 ± 3.67 356.10 ± 7.05
+AC 71.83 ± 4.20* 443.47 ± 18.17**
+BAC 63.95 ± 6.75 403.60 ± 34.30*
+Aconine 57.59 ± 8.28# 382.33 ± 29.60#
+Ver 2.40 ± 0.48*** 95.62 ± 10.12***
+Tar 7.44 ± 1.29*** 67.01 ± 2.20***
Doxorubicin (Control) 5.86 ± 0.44 94.86 ± 4.02
+AC 8.93 ± 1.86** 112.60 ± 4.47**
+BAC 6.10 ± 0.30# 105.97 ± 3.16*
+Aconine 5.58 ± 0.97# 100.46 ± 4.41#
+Ver 0.54 ± 0.03*** 1.07 ± 0.09***
+Tar 0.0033 ± 0.00035*** 0.51 ± 0.11***
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Cell viability was measured by the MTT assay. Data were expressed as the percentage of control and represent mean ± SD (n = 3). Verapamil (Ver) and tariquidar (Tar) were used as positive controls of P-gp inhibitor. *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the control group; #p < 0.05 compared with the AC group.

Reduction of acute AC toxicity by AC pretreatment

Table 2 shows the influence of 0.6 mg/kg of AC pretreatment for 14 days on the death of FVB mice caused by oral administration of AC (1.8 mg/kg). The number of deaths was 10 in the control group, but only 2 in the AC pretreatment group. The rate of mice death induced by administration of 1.8 mg/kg AC was significantly decreased from 52.6% to 10.5% (p < 0.05) after pretreatment of AC for 14 days.

Table 2. Influence of 0.6 mg/kg of AC pretreatment for 14 days on the death rate of FVB mice induced by oral administration of AC (1.8 mg/kg).

Group Pretreatment for 14 days Dosage of AC (mg/kg) Number of animal Number of death Death rate (%) p value
1 0.1% DMSO 1.8 19 10 52.6 –
2 0.6 mg/kg of AC 1.8 19 2 10.5 p < 0.05
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Discussion

Aconitum alkaloids are mainly used in China and other Asian countries to treat rheumatoid arthritis and cardiovascular diseases. However, application of Aconitum alkaloids is restricted because of their high toxicity. The current study is the first to explore the effects of three Aconitum alkaloids, namely AC, BAC, and aconine, on P-gp, and our findings are important for assessing DDIs between Aconitum alkaloids and other P-gp substrate drugs.

One of our major findings is that the tested drugs could significantly induce P-gp expression in both LS174T and Caco-2 cell lines. In LS174T cells, AC, BAC, and aconine induced P-gp levels in a dose- and time-dependent manner in the order AC > BAC > aconine (Fig. 1A,B), suggesting that P-gp induction by Aconitum alkaloids was correlated with toxicity. Based on our results and the report21, a concentration of 50 μM and a 6 day treatment period were selected to perform subsequent in vitro experiments. Rifampicin, a model inducer of P-gp20, induced P-gp expression as expected (Fig. 1C). Increase in P-gp levels were also detected in Caco-2 cells followed the order AC > BAC > aconine, after the same treatment (Fig. 1D). More importantly, AC significantly increased the P-gp protein levels in the jejunum, ileum, and colon of FVB mice (Fig. 1E). Next, we observed that AC significantly increased the MDR1 mRNA levels in both cell lines with consistent variation in the P-gp protein levels (Fig. 1F,G), suggesting transcriptional regulation of P-gp by the tested drugs. The immunostaining data for P-gp were consistent with Western blot data on P-gp levels (Fig. 2). Together, these data led us to conclude that AC could induce P-gp expression both in vitro and in vivo.

To evaluate whether NRs mediate the induction P-gp expression by the tested drugs in vitro, we performed incubations in the presence or absence of ketoconazole, an inhibitor of non-specific NR-mediated P-gp induction40. As shown in Fig. 3A,C, ketoconazole alone did not significantly modify P-gp levels, but markedly prevented P-gp expression from being induced by the tested drugs, thereby indicating that either of the NRs could mediate upregulation of P-gp expression. To investigate this further, we measured the protein levels of the main NRs and observed significant increases in PXR protein levels in both cell lines in the order AC > BAC > aconine (Fig. 3B,D). A similar trend of increase in the CAR protein levels was also found in Caco-2 cells (Fig. 3D). We could not determine CAR protein levels in the treated LS174T cell line because of its low baseline CAR levels37,41. The ligand RXRα remained unchanged. An additional study was conducted to determine the mRNA levels of the main NRs. Only a significant increase in PXR and CAR mRNA levels was observed followed the order AC > BAC > aconine (Fig. 4). Strong positive correlations were found between MDR1 mRNA induction and increase in PXR and CAR protein levels (Fig. 5), suggesting that the tested drugs induced P-gp, likely via activation of the PXR and CAR pathways.

Another major finding of this study was the functional consequences of P-gp induction in vitro. P-gp efflux activity was evaluated by the ability of the cells to carry out rhodamine 123, a typical P-gp substrate23. As shown in Fig. 6A, AC and BAC significantly increased P-gp activity in LS174T cells, which were reflected in the decreased intracellular content of rhodamine 123. Obviously, increase in P-gp activity followed the trend AC > BAC > aconine, which indicated that changes in P-gp function corresponded to changes in P-gp expression. A similar response was also observed in Caco-2 cells (Fig. 6B). Verapamil, a classical P-gp-mediated drug transport inhibitor, could reverse the decrease in rhodamine 123 accumulation in both cell lines, suggesting that the decrease was P-gp-dependent.

P-gp is an efflux pump and its activity is directly dependent on energy released by ATP hydrolysis. Thus, measurement of intracellular ATP levels is a commonly used approach to explore the mechanism of changes in P-gp function42. In both cell lines, AC and BAC significantly increased intracellular ATP levels (Fig. 7A,B). Cardiolipin is an important parameter for investigating intracellular events and a critical indicator of mitochondrial function43. The role of cardiolipin in biological membranes is to accomplish oxidative phosphorylation leading to ATP synthesis44,45. Thus, we further measured the quality of mitochondria in cells using NAO, which acts as a highly specific probe for cardiolipin43. As shown in Fig. 7C,D, the mean fluorescence intensity of NAO in cells exposed to AC significantly increased, which indicated that AC could increase mitochondrial mass. However, this response was modest or absent in the BAC or aconine groups. Taken together, these results suggest that increased intracellular ATP levels and mitochondrial mass may mediate the increase in P-gp function induced by AC and BAC.

Finally, induction of P-gp has been shown to confer resistance to a vast array of chemotherapeutic drugs because of its ability to increase drug efflux46,47. We postulated that AC could decrease the toxicity produced by chemotherapeutic agents towards cells because of its induction of P-gp. Our results showed that AC significantly increased the IC50 values related to vincristine and doxorubicin cytotoxicity relative to control cells (Table 1), which indicated that AC decreased the toxicity of the tested anticancer drugs towards the cells. In addition, pretreatment of AC could significantly reduce the rate of death of FVB mice caused by oral administration of AC (Table 2). Given these results, we speculate that AC pretreatment may protect mice against AC acute toxicity through P-gp induction in the jejunum, ileum, and colon of FVB mice, thereby decreasing AC absorption. However, it is also possible that AC can induce CYP3A4 or/and CYP2D6, which are the most important CYP isoforms responsible for AC metabolism48, thereby accelerating AC metabolism.

In summary, we have demonstrated increased P-gp expression in response to treatment with three Aconitum alkaloids in vitro and in vivo. AC produced stronger induction of P-gp than BAC and aconine. Furthermore, P-gp induction was mediated in part via NRs, and PXR and CAR are likely to be part of this mechanism. AC also activated P-gp transport activity in LS174T and Caco-2 cells, concomitantly decreasing the toxicity of vincristine and doxorubicin towards cells. In addition, AC protected mice against acute AC toxicity. We identified possible DDIs of P-gp when Aconitum alkaloids, especially AC, are co-administered with other P-gp substrates. Clinical use of Aconitum alkaloid-related therapy should be carefully monitored to avoid adverse interactions.

Materials and Methods

Materials

AC, BAC, and aconine (purity >98%) were purchased from Chengdu Mansite Pharmaceutical Co., Ltd. (Chengdu, China). Vincristine, doxorubicin and tariquidar (purity >98%) were purchased from Dalian Meilun Biological Technology Co., Ltd. (Dalian, China). Rifampicin, verapamil, rhodamine 123, dimethyl sulfoxide (DMSO), ketoconazole, phenylmethylsulfonyl fluoride, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and nonyl acridine orange (NAO) were purchased from Sigma–Aldrich (St. Louis, MO, USA). RIPA lysis buffer, BCA protein assay kit, and ATP level detection kit were purchased from Beyotime Institute of Biotechnology (Haimen, Jiangsu, China). All other chemicals and solvents were of analytical grade or better and used as received.

Experiments In Human Cell Lines

Cell lines and cell culture

The human LS174T cell line was purchased from Shanghai Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). The human Caco-2 cell line was provided by Dr. Ming Hu (Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy, University of Houston, Houston, TX, USA). LS174T and Caco-2 cells were cultured in DMEM containing 10% (v/v) fetal bovine serum, 100 U/ml penicillin, and 100 μg/ml streptomycin. The cells were grown at 37 °C in a humidified atmosphere with 5% CO2.

Cell treatments

To investigate the time-dependent effects on P-gp expression in LS174T, cells were seeded in six-well plates and incubated with AC, BAC, aconine (50 μM), or the DMSO vehicle control for 2, 4, 6, and 8 days. Cells were seeded at different densities (8 × 105, 5 × 105, 3 × 105, and 1 × 105 cells/well for 2, 4, 6, and 8 days, respectively) to avoid over-confluence. To assess the dose-dependent effects on P-gp expression following treatment with the tested drugs, cells were seeded at 3 × 105 cells/well and incubated with AC, BAC, or aconine (5, 10, 20, 50, and 100 μM) for 6 days. As positive control, LS174T cells were exposed to rifampicin (20 μM) for P-gp induction. Induction experiment was also conducted in Caco-2 cells. Caco-2 cells were seeded at 3 × 105 cells/well and incubated with AC, BAC, aconine (50 μM), or the DMSO vehicle control for 6 days. The medium was renewed on alternative days when a fresh drug or vehicle was added.

Ketoconazole was used to explore the mediation effects of NRs on P-gp expression induced by AC, BAC, and aconine. Cells were seeded at 3 × 105 cells/well in six-well plates and exposed to the vehicle (DMSO, control), ketoconazole (50 μM), AC/BAC/aconine (50 μM), or AC/BAC/aconine (50 μM) + ketoconazole (50 μM) for 6 days.

The cytotoxicity of AC, BAC, and aconine on LS174T and Caco-2 cells were evaluated using the MTT assay. Both cell lines were cultured in the 96-well plates for 24 h at a seeding density of 1.0 × 104 cells/well before the addition of drugs. After treatment with AC, BAC, and aconine at different concentrations (0, 1, 10, 50, 100, 200, or 400 μM) for different times (24, 48, 72, or 96 h), the medium was removed and 100 μl of MTT reagent (0.5 mg/ml) was added to each well for another 4 h of incubation. At the end of the incubation period, the medium was removed; intracellular formazan was solubilized with 150 μl of DMSO and quantified spectrophotometrically (λ = 570 nm) with a microplate reader Victor X3 (PerkinElmer, Waltham, MA, USA). The percentage of cell viability was calculated based on the measured absorbance relative to the absorbance of control cells. The rate of MTT conversion in all the treated groups was not statistically different from the respective control cells (Supplementary Fig. S2).

Western blot analysis

Cell lysates were prepared with RIPA lysis buffer containing 1 mM phenylmethylsulfonyl fluoride as a protease inhibitor. Protein concentrations were determined with a BCA estimation kit according to the manufacturer’s instructions. Cell protein (40 μg) was loaded onto each lane and separated by SDS-PAGE (4% stacking gel; 10% separating gel). The separated proteins were transferred from the gel to the PVDF membrane. After blocking for 1 h with non-fat milk (5%, w/v) in Tris-buffered saline containing 0.1% Tween-20 (TBST), the primary antibody of P-gp (Abcam, Cambridge, UK), PXR, CAR, RXRα, or β-actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at a dilution of 1:1000 was added to TBST with 5% non-fat milk and then incubated with the membrane at 4 °C overnight. The membrane was washed before incubation with the corresponding secondary antibody at a dilution of 1:3000 in the same buffer for 1 h at room temperature. Western blot signals were detected with an ECL chemiluminescence detection agent. The relative intensity of each protein band was scanned and quantified by Quantity One (Bio-Rad, Hercules, CA, USA).

Confocal microscopy

Both LS174T and Caco-2 cells were seeded on sterile glass cover slips in six-well plates. Cells were treated with AC, BAC, or aconine at the final concentration of 50 μM for 6 days. At the end of the treatment, cells were washed with PBS three times and fixed in 4% paraformaldehyde for 30 min. After three washes with PBS, cells were permeabilized with 0.5% Triton X-100 for 20 min and washed with PBS for another three times. Once cells were fixed and permeabilized, they were blocked with 5% bovine serum albumin for 1 h at room temperature before treatment with monoclonal anti-P-gp antibody (1:100; F4 clone, Sigma–Aldrich, St. Louis, MO, USA) at 4 °C overnight. The cells were washed with PBS and stained with secondary fluorescent antibody (1:200; Alexa Fluor 488, Santa Cruz Biotechnology, Santa Cruz, CA, USA) for 1 h at room temperature. Subsequently, cells were washed with PBS and incubated with DAPI (5 μg/ml) for another 20 min. Fluorescent signals were detected with a confocal fluorescence microscope (Leica, Germany).

Real-time PCR analysis

After cell treatment of AC, BAC, or aconine (50 μM, 6 days), total RNA was extracted by the TRIzol extraction method (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. cDNA was reverse-transcribed from total RNA with a reverse transcription kit (TaKaRa, Shiga, Japan). SYBR Green real-time PCR amplification and detection were performed using an ABI 7500 fast system (Applied Biosystems, Foster City, CA, USA). The sequences of the primers are listed in Supplementary Table S1. The thermal profile for real-time PCR was 95 °C for 30 s, 95 °C for 5 s, and 60 °C for 34 s. A melting curve was also obtained. Target mRNA levels were normalized against GAPDH mRNA levels, and all samples were run in triplicate.

Transport activity of P-gp

The efflux activity of P-gp was evaluated by the ability of the cells to detect the fluorescent compound rhodamine 123. Cells were seeded at 3 × 105 cells/well in six-well plates. After cell treatment of AC, BAC, aconine (50 μM, 6 days) or the DMSO vehicle control, the medium was replaced with fresh medium containing Rh123 (final concentration, 1 μM) with or without verapamil (50 μM and 100 μM for LS174T cells and Caco-2 cells, respectively). After 90 min of incubation at 37 °C, the cells were washed twice with cold PBS and re-suspended in PBS for further measurement. Cells that had not been exposed to rhodamine 123 were used as negative controls. The fluorescence intensity of rhodamine 123 was measured by a BD FACSAria III flow cytometer (BD Biosciences, San Jose, CA), with a 488 nm argon laser and FL1 channel. All the data were analyzed by Flow Jo 7.6.1 software (Tree Star, Inc., Ashland, OR, USA). The mean fluorescence value was converted to the percentage of control.

ATP determination

Cellular ATP levels were measured using a firefly luciferase ATP assay kit according to the manufacturer’s instructions. In brief, cells were seeded at 3 × 105 cells/well in six-well plates. After cell treatment of AC, BAC, aconine (50 μM, 6 days) or the DMSO vehicle control, the cells were washed twice with cold PBS and lysed with the ATP-releasing reagent provided by the kit. The luciferin substrate and luciferase enzyme were added, and bioluminescence was assessed by a Victor X3 spectrofluorometer (PerkinElmer, Waltham, MA, USA). Standard curves were also generated, and the protein concentration of each treatment group was determined with the BCA protein assay kit. The intracellular ATP level was normalized by the protein content in each sample (nmol/mg protein). The level of cellular ATP was converted to the percentage of control.

Detection of mitochondrial mass

Cells were seeded at 3 × 105 cells/well in six-well plates. After cell treatment of AC, BAC, aconine (50 μM, 6 days) or the DMSO vehicle control, the medium was replaced with fresh medium containing NAO (final concentration, 500 nM). After 30 min of incubation at 37 °C, the cells were washed twice with cold PBS and re-suspended in PBS for measurement. The mean fluorescence intensity of NAO was measured by a BD FACSAria III flow cytometer (BD Biosciences, San Jose, CA, USA), with a 488 nm argon laser and FL1 channel. All the data were analyzed using Flow Jo 7.6.1 software (Tree Star, Inc., Ashland, OR, USA). The mean fluorescence value was converted to the percentage of control.

Cytotoxicity assay

The MTT colorimetric assay was used to test the potential role of AC, BAC, or aconine against the cytotoxicity produced by the antitumor drugs vincristine and doxorubicin. Cells were seeded in 96-well plates at a density of 8 × 103 cells/well. After incubation at 37 °C for 24 h, cells were pretreated with AC, BAC, aconine (50 μM, 6 days) or the DMSO vehicle control and then incubated with fresh medium containing different concentrations of vincristine (0–800 μM) or doxorubicin (0–200 μM) in the presence or absence of verapamil (50 μM for LS174T cells and 100 μM for Caco-2 cells), or tariquidar (500 nM) for an additional 48 and 96 h for LS174T and Caco-2 cells, respectively. Cell viability was measured by the MTT assay, as described above. The IC50 values, which represent the concentration of vincristine or doxorubicin resulting in 50% viability, were calculated using GraphPad Prism 5.0 (GraphPad Software, La Jolla, CA, USA).

Experiments in Mice

Animals and treatments

Male FVB mice (20–22 g) were supplied by Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China; license: SCXK, Beijing, 2012-0001). The mice were kept in an environmentally controlled room (temperature: 23 °C–25 °C; relative humidity: 40%–70%; 12 h light/dark cycle) with free access to standard feed and water. The mice were randomly divided into three groups. The AC group orally received AC at 0.4 mg/kg (n = 8) and 0.6 mg/kg (n = 7) once per day over 14 consecutive days. The control group (n = 6) received only the same volume of the vehicle (0.1% DMSO) for the same time period. The animal experiments were performed in accordance with the care and use of laboratory animals and were approved by the ethics committee of Guangzhou University of Chinese Medicine (Guangzhou, China).

Specimen collection

All the mice were sacrificed by cervical dislocation after administration of AC for 14 days. The jejunum, ileum and colon were removed. The total tissue proteins were obtained by scraping and homogenized with RIPA lysis buffer containing 1 mM phenylmethylsulfonyl fluoride as a protease inhibitor. The protein concentrations were determined with the BCA estimation kit according to the manufacturer’s instructions.

Western blot analysis

The proteins in the jejunum, ileum or colon (60 μg) from different pretreatment groups were separated by SDS-PAGE. Other detailed procedures were described above. The primary antibodies of P-gp (Abcam, Cambridge, UK) or β-actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA) were used.

Determination of mortality in mice given AC after AC induction for 14 days

Male FVB mice (20–22 g) were randomly divided into two groups with 19 mice in each group. The mice were kept in the same environmentally controlled room described above. The AC group orally received AC at 0.6 mg/kg once per day over 14 consecutive days. The control group received only the same volume of the vehicle (0.1% DMSO) for the same time period. On the fifteenth day, each group of mice was orally given 1.8 mg/kg of AC. Mortality and signs of acute toxicity were monitored and recorded during a 24-period after the administration of AC. Death rate were calculated by dividing the number of death animals with the total number of animals in each group.

Statistical analysis

All the assays were performed in three independent experiments. All results were presented as mean ± standard deviation (SD). Significant differences were analyzed using Student’s t-test (two groups) or one-way ANOVA followed by the LSD post-hoc test (for more than two groups) by SPSS 19.0. Significance of death rate was analyzed using χ2 test, and correlation analyses were performed using Pearson product–moment correlation by SPSS 19.0. Statistical differences were considered significant at p < 0.05.

Additional Information

How to cite this article: Wu, J. et al. Induction of P-glycoprotein expression and activity by Aconitum alkaloids: Implication for clinical drug–drug interactions. Sci. Rep. 6, 25343; doi: 10.1038/srep25343 (2016).

Supplementary Material

Supplementary Information
srep25343-s1.pdf (451.8KB, pdf)

Acknowledgments

This project was supported by the grants of National Natural Science Foundation of China (81473410 and 81503466), the Science and Technology Project of Guangzhou City (151800014), National Basic Research Program of China (973 Program) (2011CB505305) and the Macao Science and Technology Development Fund (086/2013/A3).

Footnotes

The authors declare no competing financial interests.

Author Contributions L.L, Z.L. and L.L. designed the research; J.W., F.L., G.Z., S.H., Y.Z., R.O., N.L., S.L. and L.F. performed the study; J.W. and N.L. analyzed the data; J.W. and F.L. wrote and revised the manuscript; all authors read and approved the final version.

References

  1. Singhuber J., Zhu M., Prinz S. & Kopp B. Aconitum in traditional Chinese medicine: a valuable drug or an unpredictable risk? J Ethnopharmacol 126, 18–30 (2009). [DOI] [PubMed] [Google Scholar]
  2. Gao T., Bi H., Ma S. & Lu J. The antitumor and immunostimulating activities of water soluble polysaccharides from Radix Aconiti, Radix Aconiti Lateralis and Radix Aconiti Kusnezoffii. Prod Commun 5, 447–455 (2010). [PubMed] [Google Scholar]
  3. Lu G. et al. Toxicity assessment of nine types of decoction pieces from the daughter root of Aconitum carmichaeli (Fuzi) based on the chemical analysis of their diester diterpenoid alkaloids. Planta Med 76, 825–830 (2010). [DOI] [PubMed] [Google Scholar]
  4. Wang X. et al. Metabolomics study on the toxicity of aconite root and its processed products using ultraperformance liquid-chromatography/electrospray-ionization synapt high-definition mass spectrometry coupled with pattern recognition approach and ingenuity pathways analysis. J Proteome Res 11, 1284–1301 (2012). [DOI] [PubMed] [Google Scholar]
  5. Ye L. et al. Characterization of metabolites and human P450 isoforms involved in the microsomal metabolism of mesaconitine. Xenobiotica 41, 46–58 (2011). [DOI] [PubMed] [Google Scholar]
  6. Bao Y., Yang F. & Yang X. CE-electrochemiluminescence with ionic liquid for the facile separation and determination of diester-diterpenoid Aconitum alkaloids in traditional Chinese herbal medicine. Electrophoresis 32, 1515–1521 (2011). [DOI] [PubMed] [Google Scholar]
  7. Wada K. et al. Effects of long-term administrations of aconitine on electrocardiogram and tissue concentrations of aconitine and its metabolites in mice. Forensic Sci Int 148, 21–29 (2005). [DOI] [PubMed] [Google Scholar]
  8. Mizugaki M. et al. Quantitative analysis of Aconitum alkaloids in the urine and serum of a male attempting suicide by oral intake of aconite extract. J Anal Toxicol 22, 336–340 (1998). [DOI] [PubMed] [Google Scholar]
  9. Bisset N. G. Arrow poisons in China. Part II. Aconitum–botany, chemistry, and pharmacology. J Ethnopharmacol 4, 247–336 (1981). [DOI] [PubMed] [Google Scholar]
  10. Singh S., Fadnis P. P. & Sharma B. K. Aconite poisoning. J Assoc Physicians India 34, 825–826 (1986). [PubMed] [Google Scholar]
  11. Fenner K. S. et al. Drug-drug interactions mediated through P-glycoprotein: clinical relevance and in vitro-in vivo correlation using digoxin as a probe drug. Clin Pharmacol Ther 85, 173–181 (2009). [DOI] [PubMed] [Google Scholar]
  12. Giacomini K. M. et al. Membrane transporters in drug development. Nat Rev Drug Discov 9, 215–236 (2010). [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hu T. et al. Reversal of P-glycoprotein (P-gp) mediated multidrug resistance in colon cancer cells by cryptotanshinone and dihydrotanshinone of Salvia miltiorrhiza. Phytomedicine 21, 1264–1272 (2014). [DOI] [PubMed] [Google Scholar]
  14. Verschraagen M., Koks C. H., Schellens J. H. & Beijnen J. H. P-glycoprotein system as a determinant of drug interactions: the case of digoxin-verapamil. Pharmacol Res 40, 301–306 (1999). [DOI] [PubMed] [Google Scholar]
  15. Zhou S. F. Structure, function and regulation of P-glycoprotein and its clinical relevance in drug disposition. Xenobiotica; the fate of foreign compounds in biological systems 38, 802–832 (2008). [DOI] [PubMed] [Google Scholar]
  16. Klaassen C. D. & Aleksunes L. M. Xenobiotic, bile acid, and cholesterol transporters: function and regulation. Pharmacol Rev 62, 1–96 (2010). [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Xu C., Li C. Y. & Kong A. N. Induction of phase I, II and III drug metabolism/transport by xenobiotics. Arch Pharm Res 28, 249–268 (2005). [DOI] [PubMed] [Google Scholar]
  18. Kohle C. & Bock K. W. Coordinate regulation of human drug-metabolizing enzymes, and conjugate transporters by the Ah receptor, pregnane X receptor and constitutive androstane receptor. Biochem Pharmacol 77, 689–699 (2009). [DOI] [PubMed] [Google Scholar]
  19. Wang Y. M., Ong S. S., Chai S. C. & Chen T. Role of CAR and PXR in xenobiotic sensing and metabolism. Expert Opin Drug Metab Toxicol 8, 803–817 (2012). [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Geick A., Eichelbaum M. & Burk O. Nuclear receptor response elements mediate induction of intestinal MDR1 by rifampin. J Biol Chem 276, 14581–14587 (2001). [DOI] [PubMed] [Google Scholar]
  21. Kota B. P., Tran V. H., Allen J., Bebawy M. & Roufogalis B. D. Characterization of PXR mediated P-glycoprotein regulation in intestinal LS174T cells. Pharmacol Res 62, 426–431 (2010). [DOI] [PubMed] [Google Scholar]
  22. Mitin T., Von Moltke L. L., Court M. H. & Greenblatt D. J. Levothyroxine up-regulates P-glycoprotein independent of the pregnane X receptor. Drug Metab Dispos 32, 779–782 (2004). [DOI] [PubMed] [Google Scholar]
  23. Rigalli J. P. et al. Pregnane X receptor mediates the induction of P-glycoprotein by spironolactone in HepG2 cells. Toxicology 285, 18–24 (2011). [DOI] [PubMed] [Google Scholar]
  24. Cerveny L. et al. Valproic acid induces CYP3A4 and MDR1 gene expression by activation of constitutive androstane receptor and pregnane X receptor pathways. Drug Metab Dispos 35, 1032–1041 (2007). [DOI] [PubMed] [Google Scholar]
  25. Li Y., Wang Q., Yao X. & Li Y. Induction of CYP3A4 and MDR1 gene expression by baicalin, baicalein, chlorogenic acid, and ginsenoside Rf through constitutive androstane receptor- and pregnane X receptor-mediated pathways. Eur J Pharmacol 640, 46–54 (2010). [DOI] [PubMed] [Google Scholar]
  26. Li N. et al. Intestinal transport of pure diester-type alkaloids from an aconite extract across the Caco-2 cell monolayer model. Planta Med 78, 692–697 (2012). [DOI] [PubMed] [Google Scholar]
  27. Yang C. et al. Transcellular transport of aconitine across human intestinal Caco-2 cells. Food Chem Toxicol 57, 195–200 (2013). [DOI] [PubMed] [Google Scholar]
  28. Yang C. et al. P-glycoprotein is responsible for the poor intestinal absorption and low toxicity of oral aconitine: in vitro, in situ, in vivo and in silico studies. Toxicol Appl Pharmacol 273, 561–568 (2013). [DOI] [PubMed] [Google Scholar]
  29. Ye L. et al. The role of efflux transporters on the transport of highly toxic aconitine, mesaconitine, hypaconitine, and their hydrolysates, as determined in cultured Caco-2 and transfected MDCKII cells. Toxicol Lett 216, 86–99 (2013). [DOI] [PubMed] [Google Scholar]
  30. Zhu L. et al. The exposure of highly toxic aconitine does not significantly impact the activity and expression of cytochrome P450 3A in rats determined by a novel ultra performance liquid chromatography-tandem mass spectrometric method of a specific probe buspirone. Food Chem Toxicol 51, 396–403 (2013). [DOI] [PubMed] [Google Scholar]
  31. He D. et al. Effects of Radix Glycyrrhiza and its main component on the function and expression of P-glycoprotein in Caco-2 cells. Chinese Pharmaceutical Journal 45, 751–755 (2010). [Google Scholar]
  32. Peng Y., Tan X. & Jia X. Effects of flavonoids from Glycyrrhizae Radix on function and expression of P-glycoprotein in Caco-2 cells. Chinese Traditional and Herbal Drugs 44, 2703–2709 (2013). [Google Scholar]
  33. Hou Y. C., Lin S. P. & Chao P. D. Liquorice reduced cyclosporine bioavailability by activating P-glycoprotein and CYP 3A. Food Chem 135, 2307–2312 (2012). [DOI] [PubMed] [Google Scholar]
  34. Sadiq M. W. et al. Validation of a P-Glycoprotein (P-gp) Humanized Mouse Model by Integrating Selective Absolute Quantification of Human MDR1, Mouse Mdr1a and Mdr1b Protein Expressions with in vivo Functional Analysis for Blood-Brain Barrier Transport. PloS one 10, e0118638 (2015). [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Szakacs G., Paterson J. K., Ludwig J. A., Booth-Genthe C. & Gottesman M. M. Targeting multidrug resistance in cancer. Nat Rev Drug Discov 5, 219–234 (2006). [DOI] [PubMed] [Google Scholar]
  36. Arias A. et al. Regulation of expression and activity of multidrug resistance proteins MRP2 and MDR1 by estrogenic compounds in Caco-2 cells. Role in prevention of xenobiotic-induced cytotoxicity. Toxicology 320, 46–55 (2014). [DOI] [PubMed] [Google Scholar]
  37. Fan J., Maeng H. J., Du Y., Kwan D. & Pang K. S. Transport of 5,5-diphenylbarbituric acid and its precursors and their effect on P-gp, MRP2 and CYP3A4 in Caco-2 and LS180 cells. Eur J Pharm Sci 42, 19–29 (2011). [DOI] [PubMed] [Google Scholar]
  38. Oscarson M. et al. Effects of rifampicin on global gene expression in human small intestine. Pharmacogenet Genomics 17, 907–918 (2007). [DOI] [PubMed] [Google Scholar]
  39. Huang L. et al. Induction of P-glycoprotein and cytochrome P450 3A by HIV protease inhibitors. Drug Metab Dispos 29, 754–760 (2001). [PubMed] [Google Scholar]
  40. Huang H. et al. Inhibition of drug metabolism by blocking the activation of nuclear receptors by ketoconazole. Oncogene 26, 258–268 (2007). [DOI] [PubMed] [Google Scholar]
  41. Huang R., Murry D. J., Kolwankar D., Hall S. D. & Foster D. R. Vincristine transcriptional regulation of efflux drug transporters in carcinoma cell lines. Biochem Pharmacol 71, 1695–1704 (2006). [DOI] [PubMed] [Google Scholar]
  42. Batrakova E. V. et al. Mechanism of sensitization of MDR cancer cells by Pluronic block copolymers: Selective energy depletion. Br J Cancer 85, 1987–1997 (2001). [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Gohil V. M., Gvozdenovic-Jeremic J., Schlame M. & Greenberg M. L. Binding of 10-N-nonyl acridine orange to cardiolipin-deficient yeast cells: implications for assay of cardiolipin. Anal Biochem 343, 350–352 (2005). [DOI] [PubMed] [Google Scholar]
  44. Mileykovskaya E. et al. Cardiolipin binds nonyl acridine orange by aggregating the dye at exposed hydrophobic domains on bilayer surfaces. FEBS letters 507, 187–190 (2001). [DOI] [PubMed] [Google Scholar]
  45. Haines T. H. & Dencher N. A. Cardiolipin: a proton trap for oxidative phosphorylation. FEBS letters 528, 35–39 (2002). [DOI] [PubMed] [Google Scholar]
  46. Stavrovskaya A. A. & Stromskaya T. P. Transport proteins of the ABC family and multidrug resistance of tumor cells. Biochemistry (Mosc) 73, 592–604 (2008). [DOI] [PubMed] [Google Scholar]
  47. Eckford P. D. & Sharom F. J. ABC efflux pump-based resistance to chemotherapy drugs. Chem Rev 109, 2989–3011 (2009). [DOI] [PubMed] [Google Scholar]
  48. Tang L. et al. Involvement of CYP3A4/5 and CYP2D6 in the metabolism of aconitine using human liver microsomes and recombinant CYP450 enzymes. Toxicol Lett 202, 47–54 (2011). [DOI] [PubMed] [Google Scholar]

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Supplementary Materials

Supplementary Information
srep25343-s1.pdf (451.8KB, pdf)

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