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. 2016 Mar 10;11(3):177–183. doi: 10.1080/15592294.2016.1146852

Figure 1.

Figure 1.

Establishment and characterization of neural stem cells derived from gPS cells. (A) Establishment of NSCs from gPS cells. Scale bars = 50 μm. (B) Phase contrast images of mouse fetal brain, FB-NSCs, ESCs, ES-NSCs, gPS cells, and gPS-NSCs. Scale bars = 100 μm. (C) Expression of NSC-specific genes in the derived NSC lines. (D) Pairwise scatter plots comparing the global gene expression patterns between gPS-NSCs, FB-NSCs and ES-NSCs. (E) Heat map clustering of the global gene expression profiles of ES-NSCs, FB-NSCs, gPS-NSCs, ESCs, and gPS cells. (F) Immunocytochemistry of derived NSC cells using the markers for NSCs (Sox2 and Nestin), neurons (Tuj1), astrocytes (GFAP), and oligodendrocytes (O4). Scale bar = 20 μm.