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. 2015 Aug 10;35(17):2266–2278. doi: 10.1038/onc.2015.289

Figure 1.

Figure 1

Ectopic expression of NKX6.1 suppresses the transformation and invasive ability of cancer cells in a constitutive expression system. (a) NKX6.1 expression was analyzed by western blotting in human cervical cancer cell lines and normal cervixes using an anti-NKX6.1 antibody. β-Actin was used as an internal control. (b) Expression of NKX6.1 in HeLa and CaSki cell lines stably transfected with NKX6.1 (NKX6.1 S1 and S2) or the empty vector (Vec S1 and S2) was analyzed by western blot analysis using an anti-V5 antibody. β-Actin was used as an internal control. (c) Cell proliferation (MTS) assays were performed using HeLa and CaSki cells expressing NKX6.1 or the empty vector. The absorbance values are presented as the mean±s.e. from four independent experiments. (d) Anchorage-independent growth assays were performed using HeLa and CaSki cells expressing NKX6.1 or the empty vector. (e) Matrigel invasion assays were performed using HeLa and CaSki cells expressing NKX6.1 or the empty vector. These results are presented as the mean±s.e. from three independent experiments in triplicates (analyzed by unpaired two-tailed t-test). **P<0.01 and ***P<0.001.