Fig. 8.

Nuclear AR, c-MYC, and β-catenin are expressed in different populations in the normal human prostate. Upper Panel-IHC staining for active (i.e., N-terminal hypo-phosphorylated) nuclear β-catenin. Arrowheads indicated basal cells, which are usually negative for active nuclear β-catenin. Secretory-luminal cells are nearly universally positive for nuclear active β-catenin. Lower Left Panel-Immuno-fluorescent (IF) staining for nuclear c-MYC and DAPI counter-stain. Arrows indicate occasionally c-MYC positive basal cells; arrowheads usual c-MYC negative basal cells. Lower Middle Panel-IF staining for AR and DAPI counter-stain. Arrows indicate occasionally c-MYC positive basal cells, which are AR negative; arrowheads usual c-MYC negative basal cells, which are also AR negative. Lower Right Panel-Dual IF staining for c-MYC plus AR and DAPI counter-stain. Arrows and arrowheads are as described in left and middle panels.