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. 1992 Mar 1;89(5):1919–1923. doi: 10.1073/pnas.89.5.1919

Altering the insertional specificity of a Drosophila transposable element.

J A Kassis 1, E Noll 1, E P VanSickle 1, W F Odenwald 1, N Perrimon 1
PMCID: PMC48565  PMID: 1311855

Abstract

Vectors derived from the Drosophila P element transposon are widely used to make transgenic Drosophila. Insertion of most P-element-derived vectors is nonrandom, but they exhibit a broad specificity of target sites. During experiments to identify cis-acting regulatory elements of the Drosophila segmentation gene engrailed, we identified a fragment of engrailed DNA that, when included within a P-element vector, strikingly alters the specificity of target sites. P-element vectors that contain this fragment of engrailed regulatory DNA insert at a high frequency near genes expressed in stripes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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