Figure 5.

Mte1 and Mph1 are recruited to double-strand DNA breaks. (A) ChIP-seq analysis was performed on RAD52–FLAG, MTE1–FLAG, MTE1–FLAG mph1∆, MPH1–FLAG, and MPH1–FLAG mte1∆ cells at 0 hr and 4 hr following the induction of a specific irreparable double-strand break at the MAT locus by the HO endonuclease. ChIP enrichment scores representing the log₂ immunoprecipitate to input ratio are plotted across chromosome III for each time point. The position of the HO cut site is indicated by a dashed line. (B) Extracts from cells used in A were subjected to immunoblot analysis and probed with an antiflag antibody and an antitubulin antibody (as a loading control).