Table 2. Plasmids used in this study.
| Plasmid | Genotype | Source |
|---|---|---|
| pEGKG | GAL1prom-GST | Yeast Deletion Collection (Open Biosystems, Inc.) |
| 2µ, URA3 | ||
| pEGKG-Rod1 | GAL1prom-GST | Zhu et al. (2000) |
| 2µ, URA3 | ||
| pEGKG-Rod1315Aa | GAL1prom-GST | This study |
| 2µ, URA3 | ||
| pEGKG-Rod1447Aa | GAL1prom-GST | This study |
| 2µ, URA3 | ||
| pEGKG-Rod1641Aa | GAL1prom-GST | This study |
| 2µ, URA3 | ||
| pEGKG-Rod1706Aa | GAL1prom-GST | This study |
| 2µ, URA3 | ||
| pEGKG-Rod1720Aa | GAL1prom-GST | This study |
| 2µ, URA3 | ||
| pEGKG-Rod1781Aa | GAL1prom-GST | This study |
| 2µ, URA3 | ||
| pEGKG-Rod1447A 641Aa | GAL1prom-GST | This study |
| 2µ, URA3 | ||
| pEGKG-Rod1447A 706Aa | GAL1prom-GST | This study |
| 2µ, URA3 | ||
| pEGKG-Rod13A (Rod1447A 641A 706A)a | GAL1prom-GST | This study |
| 2µ, URA3 | ||
| pEGKG-Rod14A (Rod1315A 447A 641A 706A)a | GAL1prom-GST | This study |
| 2µ, URA3 | ||
| pEGKG-Rod15A (Rod1315A 447A 641A 706A 720A)a | GAL1prom-GST | This study |
| 2µ, URA3 | ||
| pEGKG-Rod16SA (Rod1S315A S447A S641A S706A S720A S781A)a | GAL1prom-GST | This study |
| 2μ, URA3 | ||
| pGEX6P1-Rod16SE (Rod1S315E S447E S641E S706E S720E S781E)a | GAL1prom-GST | This study |
| 2μ, URA3 | ||
| pGEX6P1-Rod1ARR (Rod11-402)a | GAL1prom-GST | This study |
| 2μ, URA3 | ||
| pGEX6P1-Rod1TAIL (Rod1403-837)a | GAL1prom-GST | This study |
| 2μ, URA3 | ||
| pGEX6P1-Rod11SA ARR (Rod11-402 S315A)a | GAL1prom-GST | This study |
| 2μ, URA3 | ||
| pGEX6P1-Rod15SA TAIL (Rod1403-837 S447A S641A S706A S720A S781A)a | GAL1prom-GST | This study |
| 2μ, URA3 | ||
| pEGKG-Rod12SA (Rod1138A 807A)a | GAL1prom-GST | This study |
| 2μ, URA3 | ||
| pEGKG-Rod12SE (Rod1138E 807E)a | GAL1prom-GST | This study |
| 2μ, URA3 | ||
| pEGKG-Rod18SA (Rod1S315A S447A S641A S706A S720A S781A 138A 807A)a | GAL1prom-GST | This study |
| 2μ, URA3 | ||
| pEGKG-Rod1PANA (pJT4954) | GAL1prom-GST | Alvaro et al. (2014) |
| 2µ, URA3 | ||
| pEGKG-Rod1PASA (pJT4955) | GAL1prom-GST | Alvaro et al. (2014) |
| 2µ, URA3 | ||
| pEGKG-Rod1PPxY-less (pJT4956) | GAL1prom-GST | Alvaro et al. (2014) |
| 2µ, URA3 | ||
| pEGKG-Rod1V/PPxY-lessa | LDB19prom | This study |
| CEN, HIS3 | ||
| pEGKG-Rod12A, V/PPxY-lessa | LDB19prom | This study |
| CEN, HIS3 | ||
| pEGKG-Rod16A, V/PPxY-lessa | LDB19prom | This study |
| CEN, HIS3 | ||
| pEGKG-Rod18A, V/PPxY-lessa | LDB19prom | This study |
| CEN, HIS3 | ||
| pEGKG-Rog3 | GAL1prom-GST | Zhu et al. (2000) |
| 2µ, URA3 | ||
| pEGKG-Rog3∆400 | GAL1prom-GST | Alvaro et al. (2014) |
| 2µ, URA3 |
a
Generated by site-directed mutagenesis (Green and Sambrook 2012b) with synthetic oligonucleotides containing the desired codon alterations (using the wild-type sequences in pRS426 vectors as the template). DNA from the corresponding gene was amplified from genomic DNA by PCR (Green and Sambrook 2012a) and then cloned into pEGKG.