Figure 5. Topographical organization of projections.

The position of the centers of mass of labelling in layers I and III of PrS, layers V-VI of PrS and PaS and layers V-VI of MEC and LEC is plotted. Each dot is color coded with respect to the rostrocaudal (A) or dorsoventral position (B) of the injections in RSC (rostral half; blue, caudal half; red, A29; yellow, A30; green). In layers I and III of PrS more caudally placed injections result in projections located more ventral compared to rostrally placed injections (A; p<0.001), while ventrally placed injections result in projections located more proximal compared to dorsally placed injections (B; p<0.001). In layers V-VI of PrS and PaS caudally placed injections result in projections located more ventral compared with rostrally placed injections (A; p<0.001), while ventral and rostrally placed injections result in projections located significantly more proximal compared with dorsally and caudally placed injections (B; ventral: p<0.001, caudal: p=0.033). In MEC and LEC layers V-VI, caudal and ventrally placed injections result in projections located significantly more ventral compared to rostral and dorsally placed injections (A; caudal: p<0.001, ventral: p=0.032), while ventrally placed injections result in projections located significantly more lateral compared to dorsally placed injections (B; p=0.020). Multiple regression was used for all statistical tests (Figure 5—source data 1–12). For flatmaps of the projection patterns see Figure 5—figure supplement 1.

DOI: http://dx.doi.org/10.7554/eLife.13925.009

Figure 5—figure supplement 1. Topographical organization of RSC-PHR projections in pups.

(A) Injections were organized into 2 x 4 groups (A29/A30 x rostrocaudal) based on the location of the injection and the average projection pattern for each group was calculated. Injections in rostral RSC (left column) resulted in labeled fibers within dorsal PrS layers I, III and V-VI, while more caudal injections (the three right columns) resulted in labeling in more ventral parts of PrS, PaS and MEC. Injections in A30 (top row) resulted in labeled fibers in distal PrS, PaS and medial MEC. In contrast, injections in A29 (bottom row) resulted in labeled fibers in more proximal parts of PrS and more lateral parts of MEC. Dorsal, ventral, proximal (prox), distal (dist), medial (med), lateral (lat) indicates dorsoventral and transverse axis of the flatmaps. (B-G) Centers of mass of the labeling resulting from each injection were calculated for PrS layers I and III (B and C), PrS and PaS layers V-VI (D and E) and MEC and LEC layers V-VI (F and G). In the plots, each injection is plotted according to its location in RSC and colorcoded according to the dorsal (blue), ventral (red), distal/medial (green) and proximal/lateral (yellow) position of the center of mass of the projection in PHR (see color maps in bottom right of each figure).

DOI: http://dx.doi.org/10.7554/eLife.13925.022