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. 2016 Mar 28;6(6):808–816. doi: 10.7150/thno.13826

Figure 2.

Figure 2

Effect of the α-amino protecting group modification on Lys-AMC for the proteolytic cleavage by CTSL. (A) Comparative CTSL assay using indicated Lys-AMC substrates either with vehicle control DMSO or CTSL inhibitor Z-FY-CHO (100 μM). (B) Verification of the substrate Fmoc-Lys(Ac)-AMC for the enzyme dependency on HDACs and CTSL and cancer selectivity. The live cell enzymatic assays were performed using HCT116, HT26, CCD841-CoN and Caco-2 cells either in the presence of vehicle control DMSO, HDAC inhibitor TSA (1 μM) or Z-FY-CHO (100 μM).