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. Author manuscript; available in PMC: 2017 Apr 19.
Published in final edited form as: Immunity. 2016 Apr 5;44(4):939–950. doi: 10.1016/j.immuni.2016.03.006

Figure 1. Identification of a VRC01-class antibody DRVIA7 from an HIV-1-infected Chinese donor DRVI01.

Figure 1

(A) The neutralizing ability of DRVI01 sera from six time points from 2005 to 2009 were tested against the global and DRVI panels of pseudoviruses. Dendrograms are shown at each time point representing the pseudovirus panels with nodes representing individual viruses colored according to neutralizing potency. (B) Single B cell sorting of CD4bs-directed monoclonal antibodies by flow cytometry. Around 10 million PBMCs from DRVI01 were incubated with cell markers and sorting probes. Seven B cells that reacted with RSC3, but not RSC3Δ371I, were then sorted into 96 well microtiter plates. FITC, fluorescein isothiocyanate. FSC-H, forward scatter height; FSC-A, forward scatter area; and SSC, side scatter area. (C) Sequence analysis of DRVIA7 and VRC01 heavy and light chains with alignment to respective germline genes. Mature antibody residues that differ from germline are colored in red. See also Table S1 and Figure S1.