Zou, J.-J., Li, X.-D., Ratnasekera, D., Wang, C., Liu, W.-X., Song, L.-F., Zhang, W.-Z., and Wu, W.-H. (2015). Arabidopsis CALCIUM-DEPENDENT PROTEIN KINASE8 and CATALASE3 function in abscisic acid-mediated signaling and H2O2 homeostasis in stomatal guard cells under drought stress. Plant Cell 27: 1445–1460.
In this article, Figure 1, Figure 6, and Supplemental Figure 6 show photographs of pots containing plants corresponding to various genotypes and treatments. To create these images, multiple photographs of different groupings of pots were taken. Several mistakes were made in the physical placement of pots for Figure 1F and Supplemental Figure 6, and the images did not clearly show how the original photographs were cropped and pieced together to create each figure. We apologize for these mistakes and present the following corrected figures, legends, and explanations.
Figure 1.
Original: Arabidopsis CPK8 Acts as a Positive Regulator in ABA- and H2O2-Mediated Stomatal Movement in Response to Drought Stress.
(F) Drought stress sensitivity of the wild type, cpk8, 8COM1, and 8OE1 in soil after water was withheld for 20 d (normal drought stress) or 22 d (severe drought stress). The experiments were repeated three times with similar results.
Supplemental Figure 6.
Original: Phenotype Observation of CPK8- and CAT3-Related Plant Materials under Drought Stress.
The Superpro:CPK8 was introduced into cat3 mutant to generate 8OE/cat3 lines. The Superpro:CAT3 was introduced into cpk8 mutant to generate 3OE/cpk8 lines. For the drought stress treatment, the photographs were taken after withholding water for 20 d.
(1) In Figure 1F of the original publication, the photograph shown in the bottom panel contained mistakenly placed pots, which confused the identity of two independent transgenic lines, 8OE1 and 8OE2, for 22-d drought treatment. In addition, the 8OE1 and 8OE2 plants appear “greener” in the figure at 22 d relative to 20 d drought treatment. This was due to a difference in camera settings and light conditions for the image taken at 22 d relative to 20 d (the images are presented unaltered) but does not affect the overall results or conclusions. Figure 1F is reproduced here, with images unaltered, but marked to show which images correspond to distinct photographs and the correct genotypes for 8OE1 and 8OE2 pots at 22-d drought. The legend has been amended accordingly and to include the number of replicate pots per treatment/genotype per experiment. The conclusions of our article are unaffected by this correction.
Figure 1.
Corrected: Arabidopsis CPK8 Acts as a Positive Regulator in ABA- and H2O2-Mediated Stomatal Movement in Response to Drought Stress.
(F) Drought stress sensitivity of the wild type, cpk8, 8COM1, 8COM2, 8OE1, and 8OE2 in soil after water was withheld for 20 d (normal drought stress) or 22 d (severe drought stress). The experiments were repeated three times with similar results; for each experiment, there were three to six pots per genotype/treatment, and a representative pot from one experiment is shown. The control and 20-d drought rows are from the same photograph taken on day 20, whereas the 22-d drought row is from a separate photograph taken on day 22 (images from different photographs separated by a red line). The plants in 22-d drought pots appear a slightly different color due to different lighting and camera settings.
(2) In Supplemental Figure 6 of the original publication, pots containing cpk8/cat3 plants (the same pots shown in Figure 6 corresponding to cpk8/cat3 control and rewatered) were mistakenly placed in the photograph, instead of pots containing the 8OE/cat3 line (control and rewatered). We apologize for this error. Here, we present a revised Supplemental Figure 6 with a different photograph (taken at the same time) showing the correct grouping of pots corresponding to the stated genotypes and treatments. We further note that some of the pots shown in this figure are the same pots that were used in the photograph presented in other figures of the original article. Thus, the Col and cpk8 control pots were also placed in the grouping for the Figure 1F photograph, and cat3 rewatered pot was also placed in the grouping for the Figure 6A photograph (i.e., they are the same pots, but different photographs). The figure legend was amended to include the number of replicate pots per treatment/genotype. The conclusions of our article are unaffected by this correction.
Supplemental Figure 6.
Corrected: Phenotype Observation of CPK8- and CAT3-Related Plant Materials under Drought Stress.
The Superpro:CPK8 was introduced into cat3 mutant to generate 8OE/cat3 lines. The Superpro:CAT3 was introduced into cpk8 mutant to generate 3OE/cpk8 lines. Plants grown on Murashige and Skoog medium for 7 d were transplanted to soil for ∼1 week with sufficient watering followed by continued watering (control) or 20 d of withholding water (drought stress) followed by two more days of drought stress and then 3 d of regular watering (rewatered). Images of control and drought stress pots are from one photograph taken after 20 d of drought, and images of rewatered pots are from a separate photograph taken after rewatering (images from different photographs separated by a red line). The experiments were repeated three times with similar results; for each experiment there were three to six pots per genotype/treatment, and a representative pot is shown.
Footnotes
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Editor’s note: the corrected figures and accompanying text were reviewed by members of The Plant Cell editorial board. Both the original and corrected figures are shown for ease of comparison. The supplemental figures PDF file available for downloading online was replaced with the corrected figure.