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. 2016 Jan 13;1(1):e00036-15. doi: 10.1128/mSphere.00036-15

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Copyright © 2016 Devigne et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 5 — Assay for the DNA negative supercoiling activity of the D. radiodurans DNA gyrase. DNA gyrase was reconstituted by mixing the GyrA and GyrB subunits in equimolar amounts. (A) Relaxed pHOT plasmid DNA was incubated with the indicated increasing concentrations of DNA gyrase. SC, supercoiled plasmid DNA; R, relaxed plasmid DNA. (B) Effect of PprA on the DNA negative supercoiling activity of DNA gyrase. Relaxed pHOT DNA was incubated with DNA gyrase (8 nM) in the presence of the indicated increasing concentrations of PprA.