TABLE 1 .

Proteins that coprecipitated with PprA::HA protein after gamma irradiation

Gene	Function	Change in protein expression fora:
		Comparison 1 (pprA::HA strain at 3.8 kG versus 0 kGy)		Comparison 2 (pprA::HA strain versus ΔpprA strain at 3.8 kGy)
		Fold change	P value	Fold change	P value
DR_0309	Elongation factor Tu	7.2	1.95 × 10−2	7.2	3.96 × 10−2
DR_2508	Hexagonally packed intermediate layer surface protein	7.0	4.97 × 10−4	2.1	8.69 × 10−2
DR_1913	DNA gyrase subunit A	4.8	6.90 × 10−2	4.8	1.12 × 10−1
DR_1983	30S ribosomal protein S1	2.9	6.88 × 10−2	2.5	2.40 × 10−1
DR_1624	RNA helicase	2.6	2.18 × 10−1	2.6	2.90 × 10−1
DR_1955	Peptide ABC transporter substrate-binding protein	2.4	2.49 × 10−2	2.4	3.25 × 10−1
DR_0607	Chaperonin groEL	2.4	2.49 × 10−2	2.4	3.25 × 10−1
DR_0906	DNA gyrase subunit B	2.0	3.27 × 10−2	2.0	4.10 × 10−1

^aD. radiodurans ΔpprA (GY12251) and pprA::HA (GY14615) bacteria were either exposed or not to gamma irradiation (3.8 kGy) and allowed to recover in fresh medium for 110 min prior to performing a coimmunoprecipitation with anti-HA antibodies. Immunoprecipitated materials were analyzed by mass spectrometry (see Table S1 in the supplemental material). The comparison between PprA-HA samples exposed to gamma irradiation (3.8 kGy, five replicates) and nonirradiated samples (0 kGy, four replicates) was performed with the Tfold module. The comparison between gamma-irradiated samples (3.8 kGy) of the strain expressing PprA::HA (five replicates) and the pprA deletion mutant (two replicates) was performed with the ACfold module using the AC test described in Audic and Claverie (66). The eight proteins detected by shotgun proteomics with a >twofold increase after irradiation (comparison 1) and with a >twofold decrease after deletion of pprA (comparison 2) are listed.