FIG 8 .

Daughter cytoskeleton completion is impaired upon loss of K40 acetylation. (A) IFA visualizing ISP1 (red), acetyl-K40–α-tubulin (green), DNA (DAPI, blue), and GFP-Cas9 (green, nuclear) in normal parasites (top) versus those lacking acetylated α-tubulin (bottom). Dotted lines encircle an individual parasites containing excess daughter buds (bottom row, arrowhead), as visualized by numerous ISP1-positive apical cap structures. (B) IFA stained for IMC3 (red), acetyl-K40–α-tubulin (green), DNA (DAPI, blue), and GFP-Cas9 (green, nuclear). Arrowheads indicate multiple IMC3-positive structures in parasites lacking K40 α-tubulin acetylation, indicative of partially formed daughter cells that failed to complete division. Scale bars, 3 μm.