Figure 7.

Modulation of eErfe, hepcidin, and Id1 by EPO in DM-treated Tmprss6 KO mice. Tmprss6 KO mice (on a mixed background) were treated with vehicle (dimethylsulfoxide), DM, and DM plus EPO. Erfe expression was analyzed by qRT-PCR in (A) BM-derived cells and mRNA expression was normalized using the erythroid marker GypA. Liver expression of the BMP-SMAD target genes Hamp (B) was measured by qRT-PCR and normalized to the housekeeping gene Hprt1. Non-heme SIC (C) was measured. A total of 3 to 4 mice per group were analyzed. Error bars indicate ± SEM. *P < .05; **P < .01. ns, nonsignificant.