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. Author manuscript; available in PMC: 2016 May 13.
Published in final edited form as: Microcirculation. 2016 May;23(4):301–310. doi: 10.1111/micc.12278

Figure 1.

Figure 1

Schematic showing the single vessel cannulation and perfusion for directly injecting FITC-anti-HS into a post-capillary venule via a theta (θ) micropipette. Upper figure shows the pre-treatment with or without 1μM S1P in 1%BSA Ringer for 20 min via a single lumen micropipette under 37°C superfusate. After pretreatment, the single lumen micropipette was changed to a θ micropipette and the superfusate temperature was gradually decreased to 4 °C. The washout (blocking) solution was first perfused into the vessel through one lumen of the θ micropipette. Then the perfusion was switched to another lumen (Dye) to perfuse FITC-anti-HS for immunolabeling the ESG of the microvessel for 2.5h. After labeling, the perfusion was back to the washout solution to wash away the free FITC-anti-HS.