Table 1. Antibodies and other reagents used for cell detection and associated catalogue information.
See methods and results for descriptions of use. Immunogen detected is listed on the leftmost column, along with the primary and secondary antibodies used in specified experiments and concentrations. Manufacturer information contains hyperlinks to websites for the products used in these experiments.
| detection of: | experiments | antibody | antibody | manufacturer & catalogue number | concentration |
|---|---|---|---|---|---|
| reelin | all | primary | mouse anti-reelin | Millipore monoclonal AB5364 clone G10 | 1:2,000 |
| secondary | Alexa 647 donkey anti-mouse | Jackson Immunoresearch 715-605-151 | 1:500 | ||
| calbindin | all except PV axon tracing | primary | rabbit anti-calbindin | Swant polyclonal antibody D-28k, number CB38 | 1:5,000 |
| secondary | Alexa 594 donkey anti-rabbit | Jackson Immunoresearch 711-585-152 | 1:500 | ||
| calbindin | PV cell axon tracing only | primary | rabbit anti-calbindin | Swant polyclonal antibody D-28k, number CB38 | 1:5,000 |
| secondary | DyLight 405 donkey anti-rabbit | Jackson Immunoresearch 711-475-152 | 1:500 | ||
| PV | all PV immunostaining | primary | goat anti-parvalbumin | Swant polyclonal antibody number PVG213 | 1:1,000 |
| secondary | Alexa 488 donkey anti-goat | Jackson Immunoresearch 705-545-147 | 1:500 | ||
| VGLUT3 | all VGLUT3 immunostaining | primary | guinea pig anti-VGLUT3 | Chemicon polyclonal antibody, AB5421 | 1:10,000 |
| secondary | Alexa 488 donkey anti-guinea pig | Jackson Immunoresearch 706-545-148 | 1:500 | ||
| VGAT | PV bouton counting only | primary | guinea pig anti-VGAT | Synaptic Systems polyclonal antibody, number 131 004 | 1:1,000 |
| secondary | Alexa 594 donkey anti-guinea-pig | Jackson Immunoresearch 706-585-148 | 1:500 | ||
| cell fills | all except PV axon tracing | primary | biocytin-filled cell | Sigma-Aldrich B4261 | 8mM |
| secondary | DyLight 405 streptavidin | Jackson Immunoresearch 016-470-084 | 1:200 | ||
| cell fills | PV cell axon tracing only | primary | biocytin-filled cell | Sigma-Aldrich B4261 | 8mM |
| secondary | Alexa 594 streptavidin | Jackson Immunoresearch 016-580-084 | 1:200 |