Table 1. Validation results for 17 candidate fusion transcripts in the osteosarcoma cell lines.
| Fusion transcript | Positive by RNA-Seq | Breakpoint PCR validation | Sanger sequencing | ||||
|---|---|---|---|---|---|---|---|
| IOR/OS15 | IOR/MOS | MHM | normal bone | osteoblast | |||
| COL1A2-MMP14 | IOR/OS15, IOR/OS18 | + | + | + | - | - | + |
| FUS-LGMN | IOR/OS15, MG-63 | + | + | - | - | - | + |
| DHFR-PABPC1 | IOR/OS15 | + | + | - | - | - | + |
| PTMA-NPM1 | IOR/OS15 | + | + | + | - | - | + |
| PMP22-ELOVL5 | IOR/OS15 | + | + | - | - | - | + |
| CDC5L-BTBD9 | IOR/OS15 | + | - | - | - | - | + |
| RAI1-CLIC5 | IOR/OS15 | + | - | - | - | - | + |
| TP53-PPRAD | IOR/OS15 | + | - | - | - | - | + |
| MRPL39-MAML3 | IOR/OS15 | + | - | - | - | - | + |
| PLXNA2-RUNX1 | IOR/OS15 | + | - | - | - | - | + |
| EIF5A-HMGN2 | IOR/OS15, MG-63, Saos-2 | + | + | + | + | + | + |
| EEF1A1-VIM | IOR/OS15, IOR/OS18, IOR/SARG | + | + | + | + | + | + |
| EIF2S1-RBM25 | IOR/OS15 | + | - | - | - | - | + |
| COL1A1-HSP90AB1 | IOR/OS15, MG-63 | + | - | + | - | - | - |
| SCO1-CLIC5 | IOR/OS15 | + | + | + | + | + | - |
| CREB3L1-HNRPA | IOR/OS15 | - | - | - | - | - | ND |
| PDZRN4-PKM2 | IOR/OS15, IOR/OS18, MHM | - | - | - | - | - | ND |
(“+” positively validated, “-” not validated, “ND” not determinable)