Figure 4. Light-induced fusion increases ICa amplitude in CaV1.3S but not in CaV1.3L channels.
(A) Schematic of the blue light-induced dimerization system (CIBN-CRY2) fused to the C-terminal of CaV1.3S channels. The same proteins were fused to the C-terminal of CaV1.3L channels (not shown in schematic). (B) Representative current records from tsA-201 cells expressing CaV1.3S-CIBN/CaV1.3S-CRY2 (left) or CaV1.3L-CIBN/CaV1.3L-CRY2 (right), before (black traces) and after (red traces) induction of channel coupling by excitation with a 30 s pulse of 488 nm light. (C) Bar plot of the averaged fold-change in ICa following 488 nm excitation in cells expressing CaV1.3S-CIBN/CaV1.3S-CRY2 (black) or CaV1.3L-CIBN/CaV1.3L-CRY2. Bars are averages of 5 cells ± SEM (*p<0.05).