Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Gαi, but not Gαo, is required for OPN up-regulation by TSH. U2OS-TSHR cells were pretreated with PTX overnight (A), GNAO and GNAI1–3 siRNAs were used to silence Gαo and Gαi1–3 for 24 hours (B) before 5-day treatment with 100-mU/mL bTSH. OPN mRNA expression was determined by RT-qPCR. The bars represent the mean ± SEM of 3 experiments with at least 2 biological replicates.
