White-gray-opaque transitions in C. albicans (BJ1097, A) and C. dubliniensis (PC35, B). Homogeneous white, gray, or opaque cells were plated on agar plates. Colony and cellular morphologies of the 3 different phenotypes (white, gray, and opaque) are shown. The colonies and cells were imaged after 5 d of growth at 25°C in 5% CO2. Scale bar, 10 μm. Phloxine B (a red dye that stains gray colonies light pink and opaque colonies red) was added at a concentration of 5 μg/mL to Lee's GlcNAc medium.