Figure 8.

LDH release of THP1-Blue cells infected with C. ulcerans. The release of lactate dehydrogenase (LDH) as a sign of host cell damage during infection of THP1-Blue™ NF-κB cells with C. ulcerans was measured using the cytotoxicity detection kit (Roche). (A) Cells were infected for 20 h with the non-pathogenic C. glutamicum ATCC 13032 and pathogenic C. ulcerans wild type strains 809 and BR-AD22 at an MOI of 1 (white bars) and 10 (gray bars). The non-pathogenic C. glutamicum had no damaging effect. However, C. ulcerans led to LDH release at MOI 1 and 10. (B) Cells were infected with C. ulcerans mutant strains ELHA1 and ELHA3 at MOI 1 and the LDH release was compared to the corresponding wild type strain BR-AD22 which was set to 100 %. Data shown are mean values of 3 independent biological replicates each performed in triplicates ± standard deviation.