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. 2016 Jan 12;7(6):7096–7109. doi: 10.18632/oncotarget.6898

Figure 3. YM155 triggers intrinsic and extrinsic apoptotic pathways.

Figure 3

A. YM155 treatment decreased survivin expression without affecting XIAP and induced cleavage of caspases. Protein expression (caspase-3, -7, -9, -8, PARP) was detected by Western Blot analysis. B. Pre-treatment of caspase inhibitors attenuates YM155-induced apoptosis. Gastric cancer cells were pre-treated with 20 μM caspase-8 inhibitor (Z-IETD-FMK) or 20 μM caspase-9 inhibitor (Z-LEHD-FMK) for 1 hour and treated with YM155 (20 nM) for another 24 hours. Apoptosis was detected by TUNEL assay. Representative photos were taken under fluorescence microscope 24 hours after the YM155 treatment. Arrows indicate apoptotic cells (green cells) (200 ×). C. Quantification of apoptotic cells. The apoptotic rates presented from B. are the means ± SD of 3 independent experiments.* P < 0.05 compared with the control group.