The authors would like to correct Fig 5. In Fig 5B, the proPO-casp1 and Tris-HCL result was mistakenly duplicated, and in the corrected figure new correct panels are mounted for proPO-casp1 and Tris-HCl.
Fig 5. Antibacterial activity and agglutination.
The number of E. coli after in vitro incubation with recombinant proPO fragments or GFP as a control, compared with a buffer control (A), was determined. All experiments were repeated at least three times (N = 3). Each bar represents the mean ± SEM, and *** P<0.001 indicates significant differences between the treatment and Tris-HCl. The data were analyzed by one-way ANOVA. E. coli observed by light microscopy after treatment with the recombinant fragments. The scale bars represent 10 μm (B). Changes in bacterial morphology after treatment with the proPO-ppA fragment as observed by SEM (C–J). After 15 min of incubation with proPO-ppA (C) or GFP (D), cell wall disruption started to appear and is reflected by longitudinal lines (black arrows in C) in the proPO-ppA treatment. The scale bars represent 1 μm. After 40 min of incubation with Tris-HCl (E), proPO-ppA (F), and GFP (G), agglutination was clearly observed in the proPO-ppA samples. The scale bars represent 10 μm. The E. coli cells were clearly distorted after proPO-ppA treatment (H–I) in contrast to the GFP treatment (J). The pictures shown in (H) and (I) were taken from different areas. The scale bars represent 1 μm.
The authors confirm that these changes do not alter their findings. The authors have provided raw, uncropped photos with indications of the cropped area for each treatment as Supporting Information.
Supporting Information
The uncropped images of E. coli agglutination after incubation with Tris-HCl, proPO-ppA fragment, proPO-casp1 fragment, proPO-casp2 fragment, and GFP as indicated. The cropped areas used for Fig 5B are indicated with red lines.
(ZIPX)
Reference
- 1. Jearaphunt M, Noonin C, Jiravanichpaisal P, Nakamura S, Tassanakajon A, Söderhäll I, et al. (2014) Caspase-1-Like Regulation of the proPO-System and Role of ppA and Caspase-1-Like Cleaved Peptides from proPO in Innate Immunity. PLoS Pathog 10(4): e1004059 doi: 10.1371/journal.ppat.1004059 [DOI] [PMC free article] [PubMed] [Google Scholar]
Associated Data
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Supplementary Materials
The uncropped images of E. coli agglutination after incubation with Tris-HCl, proPO-ppA fragment, proPO-casp1 fragment, proPO-casp2 fragment, and GFP as indicated. The cropped areas used for Fig 5B are indicated with red lines.
(ZIPX)