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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Hepatology. 2016 Mar 15;63(6):1860–1874. doi: 10.1002/hep.28472

Figure 6. CES2 knockdown increases lipogenesis by inducing SREBP-1 expression and ER stress.

Figure 6

(A) Hepatic mRNA (left panel) and protein (middle and right panels) levels in chow-fed C57BL/6 mice (n=7–8). (B) Hepa1-6 cells were infected with Ad-shLacZ or Ad-shCes2 at an MOI of 10. After 30 h, mRNA levels were quantified (n=3). (C) Newly synthesized TG ([2H]TG) in the livers of C57BL/6J mice infected with Ad-shLacZ or Ad-shCes2 (n=5). (D) Hepatic free fatty acid (FFA) level in C57BL/6J mice (n=7–8). (E) Srebp1−/− and Srebp1+/+ mice were injected i.v. with Ad-shLacZ or Ad-shCes2 (n=5–7). After 7 days, hepatic levels of TG (left panel), Acc mRNA (middle panel) and Agpat1 mRNA (right panel) were determined. (F) Hepatic protein levels in chow-fed C57BL/6J mice (n=4–6) (left 2 panels) or Western diet-fed C57BL/6J mice (n=7) (right 2 panels). *P<0.05, **P<0.01