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. 2010 Feb 24;1(3):275–283. doi: 10.1007/s13238-010-0030-1

SUMOylation of RIG-I positively regulates the type I interferon signaling

Zhiqiang Mi 1,2, Jihuan Fu 2, Yanbao Xiong 2, Hong Tang 2,✉
PMCID: PMC4875083  PMID: 21203974

Abstract

Retinoic acid-inducible gene-I (RIG-I) functions as an intracellular pattern recognition receptor (PRR) that recognizes the 5’-triphosphate moiety of single-stranded RNA viruses to initiate the innate immune response. Previous studies have shown that Lys63-linked ubiquitylation is required for RIG-I activation and the downstream anti-viral type I interferon (IFN-I) induction. Herein we reported that, RIG-I was also modified by small ubiquitin-like modifier-1 (SUMO-1). Functional analysis showed that RIG-I SUMOylation enhanced IFN-I production through increased ubiquitylation and the interaction with its downstream adaptor molecule Cardif. Our results therefore suggested that SUMOylation might serve as an additional regulatory tier for RIG-I activation and IFN-I signaling.

Electronic Supplementary Material

Supplementary material is available for this article at 10.1007/s13238-010-0030-1 and is accessible for authorized users.

Keywords: RIG-I, SUMOylation, type I interferon, innate immunity

Electronic supplementary material

13238_2010_30_MOESM1_ESM.pdf (371KB, pdf)

Supplementary material, approximately 340 KB.

Footnotes

Electronic Supplementary Material

Supplementary material is available for this article at 10.1007/s13238-010-0030-1 and is accessible for authorized users.

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13238_2010_30_MOESM1_ESM.pdf (371KB, pdf)

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