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. 2011 Jul 12;2(6):487–496. doi: 10.1007/s13238-011-1061-y

Interactomic study on interaction between lipid droplets and mitochondria

Jing Pu 1,2, Cheol Woong Ha 3, Shuyan Zhang 1, Jong Pil Jung 3, Won-Ki Huh 3,✉, Pingsheng Liu 1,✉
PMCID: PMC4875178  PMID: 21748599

Abstract

An increasing body of evidence shows that the lipid droplet, a neutral lipid storage organelle, plays a role in lipid metabolism and energy homeostasis through its interaction with mitochondria. However, the cellular functions and molecular mechanisms of the interaction remain ambiguous. Here we present data from transmission electron microscopy, fluorescence imaging, and reconstitution assays, demonstrating that lipid droplets physically contact mitochondria in vivo and in vitro. Using a bimolecular fluorescence complementation assay in Saccharomyces cerevisiae, we generated an interactomic map of protein-protein contacts of lipid droplets with mitochondria and peroxisomes. The lipid droplet proteins Erg6 and Pet10 were found to be involved in 75% of the interactions detected. Interestingly, interactions between 3 pairs of lipid metabolic enzymes were detected. Collectively, these data demonstrate that lipid droplets make physical contacts with mitochondria and peroxisomes, and reveal specific molecular interactions that suggest active participation of lipid droplets in lipid metabolism in yeast.

Electronic Supplementary Material

Supplementary material is available for this article at 10.1007/s13238-011-1061-y and is accessible for authorized users.

Keywords: peroxisomes, bimolecular fluorescence complementation assay, protein-protein interaction, lipid metabolism, Erg6

Electronic supplementary material

13238_2011_1061_MOESM1_ESM.pdf (257.8KB, pdf)

Supplementary material, approximately 257 KB.

Footnotes

These authors contributed equally to the work.

Electronic Supplementary Material

Supplementary material is available for this article at 10.1007/s13238-011-1061-y and is accessible for authorized users.

Contributor Information

Won-Ki Huh, Email: wkh@snu.ac.kr.

Pingsheng Liu, Email: pliu@ibp.ac.cn.

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Supplementary Materials

13238_2011_1061_MOESM1_ESM.pdf (257.8KB, pdf)

Supplementary material, approximately 257 KB.

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