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. 2012 Dec 8;3(12):943–949. doi: 10.1007/s13238-012-2090-x

Expression of YB-1 enhances production of murine leukemia virus vectors by stabilizing genomic viral RNA

Wei Li 1,2, Xinlu Wang 1, Guangxia Gao 1,✉
PMCID: PMC4875382  PMID: 23225179

Abstract

Murine leukemia virus (MLV)-based retroviral vectors is widely used for gene transfer and basic research, and production of high-titer retroviral vectors is very important. Here we report that expression of the Y-box binding protein 1 (YB-1) enhanced the production of infectious MLV vectors. YB-1 specifically increased the stability of viral genomic RNA in virus-producing cells, and thus increasing viral RNA levels in both producer cells and virion particles. The viral element responsive to YB-1 was mapped to the repeat sequence (R region) in MLV genomic RNA. These results identified YB-1 as a MLV mRNA stabilizer, which can be used for improving production of MLV vectors.

Keywords: Y-box binding protein 1, retroviral vector, RNA stability

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