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. 2012 Aug 4;3(7):545–558. doi: 10.1007/s13238-012-2054-1

The growth of B cell receptor microcluster is a universal response of B cells encountering antigens with different motion features

Zhengpeng Wan 1, Wanli Liu 1,✉
PMCID: PMC4875388  PMID: 22773344

Abstract

B lymphocyte cell senses and acquires foreign antigens through clonal distributed B cell receptors (BCRs) expressed on the surface of plasma membrane. The presentation formats of antigens are quite diverse. Based on their Brownian diffusion mobility, there are three forms: free mobile soluble antigens, lateral mobile membrane bound antigens, and fixed immobile antigens. Here, using high resolution high speed live cell imaging approaches, we provide evidence that BCR microclusters are formed on the surface of B cells shortly after B cell’s encountering of antigens with each format of motion features. Through high speed live cell imaging, we determine that these BCR microclusters show dynamic growth feature and by doing so function as the basic platforms for B cells to acquire the antigens. We propose that the formation and dynamic growth of BCR microcluster is a universal mechanism for B cell to response to antigens with diverse motion features.

Electronic Supplementary Material

The online version of this article (doi:10.1007/s13238-012-2054-1 contains supplementary material, which is available to authorized users.

Keywords: BCR microcluster, TIRFM, live cell imaging, fixed immobile antigen, lateral mobile antigen, free mobile antigen

Electronic Supplementary Material

Below is the link to the electronic supplementary material.

Supplementary Info(DOCX 14 kb) (13.8KB, docx)
Video 1(AVI 1623 kb) (1.6MB, avi)
Video 2(AVI 2375 kb) (2.3MB, avi)
Video 3(AVI 840 kb) (839.5KB, avi)
Video 4(AVI 1979 kb) (1.9MB, avi)
Video 5(AVI 2264 kb) (2.2MB, avi)

Footnotes

Electronic Supplementary Material

The online version of this article (doi:10.1007/s13238-012-2054-1 contains supplementary material, which is available to authorized users.

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Supplementary Info(DOCX 14 kb) (13.8KB, docx)
Video 1(AVI 1623 kb) (1.6MB, avi)
Video 2(AVI 2375 kb) (2.3MB, avi)
Video 3(AVI 840 kb) (839.5KB, avi)
Video 4(AVI 1979 kb) (1.9MB, avi)
Video 5(AVI 2264 kb) (2.2MB, avi)

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