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. 2013 Nov 7;4(12):932–941. doi: 10.1007/s13238-013-3092-z

Microvesicle-delivery miR-150 promotes tumorigenesis by up-regulating VEGF, and the neutralization of miR-150 attenuate tumor development

Yuchen Liu 1, Luming Zhao 1, Dameng Li 1, Yuan Yin 2, Chen-Yu Zhang 1,✉, Jing Li 1,✉, Yujing Zhang 1,✉
PMCID: PMC4875402  PMID: 24203759

Abstract

Tumor-associated macrophages (TAMs) mostly exhibit M2-like (alternatively activated) properties and play positive roles in angiogenesis and tumorigenesis. Vascular endothelial growth factor (VEGF) is a key angiogenic factor. During tumor development, TAMs secrete VEGF and other factors to promote angiogenesis; thus, anti-treatment against TAMs and VEGF can repress cancer development, which has been demonstrated in clinical trials and on an experimental level. In the present work, we show that miR-150 is an oncomir because of its promotional effect on VEGF. MiR-150 targets TAMs to up-regulate their secretion of VEGF in vitro. With the utilization of cell-derived vesicles, named microvesicles (MVs), we transferred antisense RNA targeted to miR-150 into mice and found that the neutralization of miR-150 down-regulates miR-150 and VEGF levels in vivo and attenuates angiogenesis. Therefore, we proposed the therapeutic potential of neutralizing miR-150 to treat cancer and demonstrated a novel, natural, microvesicle-based method for the transfer of nucleic acids.

Electronic Supplementary Material

Supplementary material is available for this article at 10.1007/s13238-013-3092-z and is accessible for authorized users.

Keywords: microvesicle, miR-150, tumorigenesis, VEGF, neutralization, attenuation

Electronic supplementary material

13238_2013_3092_MOESM1_ESM.pdf (58.3KB, pdf)

Supplementary material, approximately 58.3 KB.

Footnotes

Electronic Supplementary Material

Supplementary material is available for this article at 10.1007/s13238-013-3092-z and is accessible for authorized users.

Contributor Information

Chen-Yu Zhang, Email: yjzhang@nju.edu.cn.

Jing Li, Email: jingli220@nju.edu.cn.

Yujing Zhang, Email: cyzhang@nju.edu.cn.

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Supplementary Materials

13238_2013_3092_MOESM1_ESM.pdf (58.3KB, pdf)

Supplementary material, approximately 58.3 KB.

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