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. 2012 Mar 17;3(2):84–88. doi: 10.1007/s13238-012-2012-y

Emerging technology of in situ cell free expression protein microarrays

Amita Nand 1, Anju Gautam 2, Javier Batista Pérez 1, Alejandro Merino 1, Jinsong Zhu 1,✉
PMCID: PMC4875412  PMID: 22426976

Abstract

Recently, in situ protein microarrays have been developed for large scale analysis and high throughput studies of proteins. In situ protein microarrays produce proteins directly on the solid surface from pre-arrayed DNA or RNA. The advances in in situ protein microarrays are exemplified by the ease of cDNA cloning and cell free protein expression. These technologies can evaluate, validate and monitor protein in a cost effective manner and address the issue of a high quality protein supply to use in the array. Here we review the importance of recently employed methods: PISA (protein in situ array), DAPA (DNA array to protein array), NAPPA (nucleic acid programmable protein array) and TUSTER microarrays and the role of these methods in proteomics.

Footnotes

An erratum to this article can be found at http://dx.doi.org/10.1007/s13238-012-2803-1.

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