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. 2012 Apr 10;3(3):213–224. doi: 10.1007/s13238-012-0014-4

System approaches reveal the molecular networks involved in neural stem cell differentiation

Kai Wang 1,5, Haifeng Wang 2,5, Jiao Wang 2,5, Yuqiong Xie 1,5, Jun Chen 3,4, Huang Yan 1,5, Zengrong Liu 5, Tieqiao Wen 1,5,✉
PMCID: PMC4875420  PMID: 22492180

Abstract

The self-renewal and multipotent potentials in neural stem cells (NSCs) maintain the normal physiological functions of central nervous system (CNS). The abnormal differentiation of NSCs would lead to CNS disorders. However, the mechanisms of how NSCs differentiate into astrocytes, oligodendrocytes (OLs) and neurons are still unclear, which is mainly due to the complexity of differentiation processes and the limitation of the cell separation method. In this study, we modeled the dynamics of neural cell interactions in a systemic approach by mining the high-throughput genomic and proteomic data, and identified 8615 genes that are involved in various biological processes and functions with significant changes during the differentiation processes. A total of 1559 genes are specifically expressed in neural cells, in which 242 genes are NSC specific, 215 are astrocyte specific, 551 are OL specific, and 563 are neuron specific. In addition, we proposed 57 transcriptional regulators specifically expressed in NSCs may play essential roles in the development courses. These findings provide more comprehensive analysis for better understanding the endogenous mechanisms of NSC fate determination.

Electronic Supplementary Material

Supplementary material is available for this article at 10.1007/s13238-012-0014-4 and is accessible for authorized users.

Keywords: neural stem cell, cell differentiation, molecular networks

Electronic supplementary material

13238_2012_14_MOESM1_ESM.pdf (271.5KB, pdf)

Supplementary material, approximately 271 KB.

13238_2012_14_MOESM2_ESM.pdf (184.2KB, pdf)

Supplementary material, approximately 184 KB.

13238_2012_14_MOESM3_ESM.pdf (1.5MB, pdf)

Supplementary material, approximately 1.52 MB.

13238_2012_14_MOESM4_ESM.pdf (729.3KB, pdf)

Supplementary material, approximately 729 KB.

13238_2012_14_MOESM5_ESM.pdf (9.8MB, pdf)

Supplementary material, approximately 9.81 KB.

Footnotes

These authors contributed equally to the work.

Electronic Supplementary Material

Supplementary material is available for this article at 10.1007/s13238-012-0014-4 and is accessible for authorized users.

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Supplementary Materials

13238_2012_14_MOESM1_ESM.pdf (271.5KB, pdf)

Supplementary material, approximately 271 KB.

13238_2012_14_MOESM2_ESM.pdf (184.2KB, pdf)

Supplementary material, approximately 184 KB.

13238_2012_14_MOESM3_ESM.pdf (1.5MB, pdf)

Supplementary material, approximately 1.52 MB.

13238_2012_14_MOESM4_ESM.pdf (729.3KB, pdf)

Supplementary material, approximately 729 KB.

13238_2012_14_MOESM5_ESM.pdf (9.8MB, pdf)

Supplementary material, approximately 9.81 KB.

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