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. 2013 Nov 10;4(11):872–881. doi: 10.1007/s13238-013-3062-5

CD1dhiCD5+ B cells differentiate into antibody-secreting cells under the stimulation with calreticulin fragment

Tengteng Zhang 1, Yun Xia 1, Lijuan Zhang 1, Wanrong Bao 1, Chao Hong 1,✉, Xiao-Ming Gao 1,✉
PMCID: PMC4875449  PMID: 24214877

Abstract

Calreticulin (CRT) is a multifunctional molecule in both intracellular and extracellular environment. We have previously found that a recombinant CRT fragment (rCRT/39-272) could modulate T cell-mediated immunity in mice via activation and expansion of CD1dhiCD5+ B cells as well as induction of CRT-specific regulatory antibodies. Antibody secreting cells (ASCs) are terminally differentiated B cells responsible for producing antibodies to participate in positive immune response as well as immune regulation. In this study, we demonstrate that rCRT/39-272 differentiates murine CD1dhiCD5+ B cells into ASCs marked by increased expression of plasma cell-associated transcription factors and production of polyreactive antibodies against DNA and CRT in vitro. Intraperitoneal administration of rCRT/39-272 augmented differentiation of CD1dhiCD5+ B cells into ASCs in naïve mice or mice with experimental autoimmune encephalomyelitis. Thus, we propose that ASC differentiation and subsequent antibody production of CD1dhiCD5+ B cells are key steps in CRT-mediated immunoregulation on inflammatory T cell responses.

Keywords: calreticulin, regulatory B cells, antibody secreting cells

Footnotes

These authors contributed equally to the work.

Contributor Information

Chao Hong, Email: chaohong@suda.edu.cn.

Xiao-Ming Gao, Email: xmgao@suda.edu.cn.

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